產品英文名稱：RoboSep™ Human Whole Blood CD34 Positive Selection Kit with Filter Tips
產品中文名稱：RoboSep™ 帶有過濾嘴的人血CD34陽性選擇試劑盒

RoboSep™ Human Whole Blood CD34 Positive Selection Kit with Filter Tips產品英文描述
RoboSep™ Human Whole Blood CD34 Positive Selection Kit with Filter Tips is designed to isolate CD34+ cells from fresh whole blood or freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells. If isolating CD34+ cells from fresh cord blod, we recommend using the EasySep™ Human Cord Blood CD34 Positive Selection Kit (Catalog #18096). If isolating CD34+ cells from any other sample type, we recommend using the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056).

RoboSep™ 帶有過濾嘴的人血CD34陽性選擇試劑盒產品組分
• EasySep™ Human Whole Blood CD34 Positive Selection Cocktail, 3 x 1.0 mL
• EasySep™ Magnetic Particles, 3 x 1.0 mL
• EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL

RoboSep™ Human Whole Blood CD34 Positive Selection Kit with Filter Tips 推薦使用
The isolation of human CD34+ cells from fresh whole blood or freshly prepared buffy coat by positive selection

RoboSep™ 帶有過濾嘴的人血CD34陽性選擇試劑盒保存方法
EasySep™ Human Whole Blood CD34 Positive Selection Cocktail
Stable at 4°C for 2 years. Do not freeze this product. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep™ Magnetic Nanoparticles EasySep
Stable at 4°C for 2 years. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep™ RBC Lysis Buffer 10X Concentrate EasySep
10X concentrate is stable at room temperature for 2 years. 1X lysis buffer is stable at 4°C for 3 months. Do not freeze.

RoboSep™ Human Whole Blood CD34 Positive Selection Kit with Filter Tips 實驗資料
FACS Profile Results with EasySep® Human Buffy Coat/Whole Blood CD34 Positive Selection Kit

Starting with buffy coat, the CD34+ cell content of the enriched fraction typically ranges from 51.7% - 90.3% (reported as a percentage of viable CD45+ cells).

Typical EasySep™ Procedure for Human Cells

RoboSep™ 帶有過濾嘴的人血CD34陽性選擇試劑盒相關產品訂購信息
Stemcell  10513  CD34 Antibody, Clone 8G12, PE-Conjugated  Mouse Monoclonal Antibody to Human CD34 - PE Conjugated 
Stemcell  10413  CD34 Antibody, Clone 8G12, FITC-Conjugated  Mouse Monoclonal Antibody to Human CD34 - FITC Conjugated 
Stemcell  10613  CD34 Antibody, Clone 8G12, APC-Conjugated  Mouse Monoclonal Antibody to Human CD34 - PE Conjugated 
Stemcell  07801  Lymphoprep™  Density Gradient Medium for the Isolation of Mononuclear Cells 
Stemcell  20110  EasySep™ Red Blood Cell Lysis Buffer  Red Blood Cell Lysis Buffer 
Stemcell  07800  Ammonium Chloride Solution  Reagent for the Lysis of Red Blood Cells 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  04034  MethoCult™ H4034 Optimum  Methylcellulose-Based Medium with Recombinant Cytokines and EPO for Human Cells 
Stemcell  04435  MethoCult™ H4435 Enriched  Methylcellulose Medium with Recombinant Cytokines and EPO for Human Cells 
Stemcell  04434  MethoCult™ H4434 Classic  Methylcellulose-Based Medium with Recombinant Cytokines for Human Cells
Stemcell  60013  Anti-Human CD34 Antibody, Clone 581  Mouse Monoclonal Antibody Against Human CD34          
For research use only. Not intended for human or animal diagnostic or therapeutic uses.