產(chǎn)品英文名稱(chēng)：Rat IFN-γ ELISPOT Set
產(chǎn)品中文名稱(chēng)：大鼠干擾素-γ ELISPOT set
Rat IFN-γ ELISPOT Set 產(chǎn)品介紹
The enzyme-linked immunospot (ELISPOT) assay is a powerful too for detectinand enumerating individual cells that secrete a particular protein in vitro.1 Based on the sandwich enzyme-linked immunosorbent assay (ELISA), the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells,2,3 the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines.4,5,6 The sensitivity of the assay lends itself to measurement of even very low frequencies of analyte-producing cells (eg, 1/300,000).1 Recent developments in assay plate design and in ELISPOT plate-reader instrumentation have significantly improved the utility of the ELISPOT method for objective and rapid analysis ofanalyte-producing cells.
大鼠干擾素-γ ELISPOT set 的成分
• Capture Antibody: Anti-rat IFN-γ
• Detection Antibody: Biotinylated anti-rat IFN-γ
• Enzyme Reagent: Streptavidin-horseradish peroxidase conjugate (SAv-HRP)
• Standard: Recombinant rat IFN-γ (lyophilized)
大鼠干擾素-γ ELISPOT set 的優(yōu)越性
•高靈敏度
•高通量，高含量的分析
•最小體積的生物材料的要求
•適用于冷凍/解凍的生物樣品 •與其他檢驗(yàn)方法兼容。例如，細(xì)胞分析。BD™ ELISPOT可以轉(zhuǎn)移克隆，增殖實(shí)驗(yàn)，流式細(xì)胞術(shù)或其他分析方法。
Rat IFN-γ ELISPOT Set 保存方法
1. Store unopened reagents at 2-8°C. Do not use reagents after expiration date, or if turbidity is evident.
2. Before use, bring all reagents to room temperature (18-25°C).Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until expiration date. See below for reconstituted standard storage information.
大鼠干擾素-γ ELISPOT set 使用注意事項(xiàng)
1. Reagents which contain preservatives may be toxic if ingested, inhaled, or in contact with skin.
2. Handle all serum and plasma specimens in accordance with NCCLS guidelines for preventing transmission of blood-borne infections.
3. Capture Antibody contains < 0.1% sodium azide. Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Detection Antibody contains BSA and ProClin.-150 as a preservative.
5. Enzyme Reagent contains BSA and ProClin.-150 as a preservative.
6. Source of all serum proteins is from USDA inspected abattoirs located in the United States
