Gibco? PSC Neural Induction Medium (prototype)
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英文名稱:Gibco? PSC Neural Induction Medium (prototype)
中文名稱:Gibco? PSC神經(jīng)誘導培養(yǎng)基(prototype)
Gibco? PSC神經(jīng)誘導培養(yǎng)基 產(chǎn)品介紹:
Gibco? PSC神經(jīng)誘導培養(yǎng)基是一種無血清培養(yǎng)基,可高效誘導人多能干細胞(PSCs)(7天)分化為神經(jīng)干細胞(NSCs)。適用培養(yǎng)的細胞源包括誘導多能干細胞、多能干細胞、胚胎干細胞和神經(jīng)細胞等。與現(xiàn)有的方法不同,使用Gibco? PSC神經(jīng)誘導培養(yǎng)基時不需要經(jīng)過形成胚狀體(EB)中間環(huán)節(jié),因此可節(jié)約時間和精力,不會引發(fā)變異。使用Gibco? PSC神經(jīng)誘導培養(yǎng)基培養(yǎng)成的神經(jīng)干細胞(NSCs)具有高效表達NSC標志物 (Sox1, Sox2, and Nestin),同時可進一步分化成其它神經(jīng)細胞類型。”prototype” 形式的培養(yǎng)基產(chǎn)品意味著這些產(chǎn)品通常都依照cGMP進行過實時定量研究。
產(chǎn)品組份:
? Neurobasal? Medium 490 mL
? Gibco? Neural Induction Supplement 10 mL
產(chǎn)品特性:
? 快速誘導NSCs:7天內(nèi)完成高效神經(jīng)誘導,無需EB
? 大量生成NSCs:100萬PSCs可生成2000萬NSCs
? 高質(zhì)量的NSCs:收獲的NSCs可冷凍保藏、擴增和分化
Gibco? PSC Neural Induction Medium (prototype) :
Rapid Induction of NSCs: Gibco? PSC Neural Induction Medium simplifies the NSC generation workflow by eliminating the need to form EBs or rosettes. By removing this step, Gibco? PSC Neural Induction Medium enables a more reproducible process with high efficiency neural induction in only 7 days.
Scalable Production of NSCs: Gibco? PSC Neural Induction Medium is a robust medium that has been shown to generate over 20 million NSCs from 1 million PSCs. The medium has been tested with multiple hPSC lines including hiPSCs derived from use of Episomal iPSC Reprogramming Vectors and the CytoTune?-iPS Sendai Reprogramming Kit.
Generation of High Quality NSCs: Neural stem cells generated using Gibco? PSC Neural Induction Medium have >80% expression of Sox1, Sox2, and Nestin (NSC markers), and <1% expression of Oct4 (pluripotency marker). In addition, expanded NSCs maintained phenotype, exhibited normal karyotype, and could be differentiated into neurons, astrocytes, and oligodendrocytes
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