產(chǎn)品英文名稱：PYROGENT?–5000 Kinetic Turbidimetric LAL Assays
產(chǎn)品中文名稱：PYROGENT?–5000動(dòng)態(tài)濁度法內(nèi)毒素檢測(cè)試劑盒
PYROGENT?–5000 Kinetic Turbidimetric LAL Assays產(chǎn)品介紹：
PYROGENT -5000產(chǎn)品適合用于實(shí)驗(yàn)室處理大量樣品，可以定量的動(dòng)態(tài)檢測(cè)革蘭氏陰性細(xì)菌內(nèi)毒素。檢測(cè)在96孔板上進(jìn)行，允許許多樣品同時(shí)檢測(cè)。The PYROGENT?–5000 assay是水樣，大量注射用藥物和醫(yī)療設(shè)備沖洗水內(nèi)毒素檢測(cè)的理想選擇。檢測(cè)使用溫育板讀數(shù)器340nm讀數(shù)，檢測(cè)靈敏度在0.01 to 100.0 EU/ml。
檢測(cè)原理：樣品和LAL（鱟變形細(xì)胞裂物）反應(yīng)物混合，革蘭氏陰性細(xì)菌內(nèi)毒素催化LAL酶原反應(yīng)，反應(yīng)初速度決定于內(nèi)毒素濃度。激活的凝固酶原水解LAL凝固蛋白酶原特定化學(xué)鍵形成凝固蛋白。水解形成的凝固蛋白會(huì)立即自我凝集成膠狀物。LAL濁度法檢測(cè)可以測(cè)定濁度增加值（光密度），濁度變化在凝膠形成之前出現(xiàn)。反應(yīng)物變混濁所需時(shí)間（反應(yīng)時(shí)間）與細(xì)菌內(nèi)毒素含量成反比例。也就是說，如果有大量?jī)?nèi)毒素存在，則反應(yīng)迅速，很快就會(huì)出現(xiàn)混濁；如果有少量?jī)?nèi)毒素存在反應(yīng)時(shí)間就會(huì)延長(zhǎng)。未知樣品內(nèi)毒素濃度可通過標(biāo)準(zhǔn)曲線計(jì)算出。
注意事項(xiàng)：
? 本品為體外診斷試劑，嚴(yán)禁用于人體內(nèi)毒素檢測(cè)
? 本品用于藥品的G-細(xì)菌內(nèi)毒素檢測(cè)時(shí)，應(yīng)遵循USP藥典細(xì)菌內(nèi)毒素檢測(cè)法的規(guī)定
? 如果說明書下載不了或者有其它產(chǎn)品信息問題可以電話咨詢
PYROGENT?–5000 Kinetic Turbidimetric LAL Assays英文介紹：
The PYROGENT?–5000 assay is perfect for laboratories needing to process large numbers of samples. This assay is performed in a 96-well plate allowing many samples to be processed at one time. It is ideal for water samples, large volume parenterals and water rinse from medical devices. The PYROGENT?–5000 assay is measured at the 340 nm wavelength and has a sensitivity range from 0.01 to 100.0 EU/ml. An incubating plate reader is required to run this assay.
PYROGENT -5000 is a quantitative, kinetic assay for the detection of Gram negative bacterial endotoxin. A sample is mixed with the reconstituted LAL reagent, placed in the photometer, and automatically monitored over time for the appearance of turbidity. The time required before the appearance of turbidity (Reaction Time) is inversely proportional to the amount of endotoxin present. That is, in the presence of a large amount of endotoxin the reaction occurs rapidly; in the presence of a smaller amount of endotoxin the reaction time is increased. The concentration of endotoxin in unknown samples can be calculated from a standard curve.
?訂購(gòu)信息：
N383(Cat.No.)? 2瓶/盒(鱟試劑)?? 100Tests?? 5.2ml/瓶(需混合的LAL重配緩沖劑體積)
N384(Cat.No.)? 2瓶/盒(鱟試劑)?? 200Tests?? 10.4ml/瓶(LAL重配緩沖劑體積)
N588(Cat.No.)? 45瓶/盒(鱟試劑)? 2250Tests? 5.2ml/瓶(LAL重配緩沖劑體積)
N688(Cat.No.)? 45瓶/盒(鱟試劑)? 4500Tests? 10.4ml/瓶(LAL重配緩沖劑體積)
