產(chǎn)品英文名稱(chēng)：R-spondin-1 Fc fusion, human
產(chǎn)品中文名稱(chēng)：人重組R-spondin-1 Fc fusion蛋白
R-spondin-1 Fc fusion, human產(chǎn)品介紹：
R-spondin-1是權(quán)威WNT信號(hào)通路的天然增強(qiáng)劑。當(dāng)與激活β-catenin信號(hào)通路的WNT蛋白聯(lián)合使用時(shí)，R-spondin-1能通過(guò)與LGR5和LGR4融合而有效提高權(quán)威WNT蛋白的活性。
向小鼠注射重組R-spondin-1蛋白可以激活β-catenin信號(hào)通路和腸道下皮細(xì)胞的增殖，為臨床改善化療誘導(dǎo)結(jié)腸炎操作提供了基礎(chǔ)和依據(jù)。
本產(chǎn)品是全片段R-spondin-1與人IgG1細(xì)胞Fc片段的C-終端相融合而得到的。這種融合保證了體內(nèi)外蛋白的穩(wěn)定性而不影響其生物活性。StemRD公司的R-spondin-1 Fc融合蛋白是由人293細(xì)胞作為分泌蛋白，并經(jīng)過(guò)一系列的色譜分析法純化而得到。
R-spondin-1 Fc fusion, human產(chǎn)品英文簡(jiǎn)述：
R-spondin-1 is a natural enhancer of the canonical WNT pathway. When used together with WNT proteins that activate the beta-catenin pathway, Rspondin- 1 enhances the activity of canonical WNT proteins by binding to LGR5 and LGR4 (refs.) Injection of recombinant R-Spondin-1 into mouse causes activation of the-catenin pathway and proliferation of intestinal crypt cells, which forms the basis for a clinical trial in amelioration of chemotherapy-induced colitis.
This product is the full-length R-Spondin-1 fused at its C-terminus to the Fc domain of human IgG1. This fusion increases the stability of the protein in vitro and in vivo without compromising its biological activity. StemRD’s R-Spondin-1 Fc fusion protein is produced in human 293 cells as a secreted protein and purified by protein A chromatography.
• Purity: Greater than 90% as determined by SDS-PAGE analysis
• Biological Activity: The activity was determined by using a TCF reporter gene assay in cultured
human cells. The EC50 ranges from 5 - 20 ng/ml in the presence of 10ng/mL human WNT-3a. Activity in other assays should be determined by each individual setting.
• For Research Use Only. Not for Use in Human
