產(chǎn)品英文名稱：Noggin,human
產(chǎn)品中文名稱：人Noggin蛋白
Noggin,human 產(chǎn)品介紹：
Noggin 被認(rèn)為是同時(shí)對(duì)BMP及抗BMP生物活性物質(zhì)均具有高親和性的結(jié)合蛋白中的一種。在胚胎干細(xì)胞培養(yǎng)過(guò)程中加入Noggin，可以有效提高干細(xì)胞的多能性。
重組的人Noggin蛋白在進(jìn)行移動(dòng)單一多肽鏈處理后共包含216個(gè)氨基酸，并且達(dá)到預(yù)測(cè)的分子量24.6kDa。 糖基化反應(yīng)后，隨著反應(yīng)條件的變化，Noggin逐漸集結(jié)成SDS-PAGE從而達(dá)到大約30kDa的分子量。
StemRD公司生產(chǎn)的Noggin來(lái)源于經(jīng)過(guò)翻譯后特異性修飾的人293細(xì)胞，且經(jīng)過(guò)一系列的色譜分析方法對(duì)產(chǎn)品進(jìn)行純化。
Noggin,human產(chǎn)品特性：
» SDS-PAGE檢測(cè)純度大于95%
» 生物活性在人293細(xì)胞中用受體基因檢測(cè)確定，EC50在25 - 100 ng/mL之間
» Noggin,human只限于科學(xué)研究，非人體使用
Noggin,human 產(chǎn)品英文簡(jiǎn)述：
Noggin has been shown to be a high-affinity binding protein to BMP and antagonizes BMP bio-activities. It can be added to cell culture medium for embryonic stem cells to maintain pluripotency.
The recombinant human Noggin comprises 216 amino acids after removal of the signal peptide and has a predicted molecular mass of 24.6 kDa. As aresult of glycosylation, it migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
StemRD's Noggin is made in human 293 cells, which renders humanspecific post-translational modifications. We purified this product through a series of chromatography.
• Purity：Greater than 95% by SDS-PAGE
• Biological Activity：The activity was determined by using a reporter gene assay in human 293 cells for the inhibition of activity of BMP4 (2.5ng/mL). The EC50 ranges from 25 - 100 ng/mL
• For Research Use Only. Not for Use in Humans
