產(chǎn)品英文名稱(chēng)：Follistatin Fc Fusion, huamn
產(chǎn)品中文名稱(chēng)：人Follistatin Fc Fusion分泌蛋白
Follistatin Fc Fusion, huamn產(chǎn)品介紹：
Follistatin是一類(lèi)與某些TGF-β家族成員相關(guān)并阻止其與相關(guān)受體相聯(lián)系的分泌蛋白。除了是Activins的天然拮抗物外，F(xiàn)ollistatin可能會(huì)抑制其它諸如BMPs，Myostatin，GDF-11和TGF-β1等TGF-β配合基的生物活性。
StemRD的Follistatin Fc融合蛋白是由全片段的人Follistatin-288與N-端基的人IgG1 Fc片段融合而產(chǎn)生的。這種融合保證了體內(nèi)外蛋白的穩(wěn)定性，以及重組蛋白二聚體對(duì)Activins的高親和性。
用無(wú)血清無(wú)蛋白的培養(yǎng)基培養(yǎng)人293細(xì)胞，看其基因表達(dá)情況。使用蛋白A色譜分析法對(duì)特定培養(yǎng)基條件下的融合蛋白進(jìn)行純化。
Follistatin Fc Fusion, huamn產(chǎn)品特性：
» Follistatin Fc Fusion, huamn經(jīng)SDS-PAGE鑒定純度大于95%
» 生物活性在培養(yǎng)人體細(xì)胞中使用SMAD受體基因檢測(cè)確定，在2ng/mL激活素A活性抑制下IC50達(dá)20 - 100 ng/ml
» 本產(chǎn)品只限于科學(xué)研究，非人體使用
Follistatin Fc Fusion, huamn產(chǎn)品英文簡(jiǎn)述：
Follistatin is a secreted protein that binds to some members of the TGF-β family and blocks their access to corresponding receptors. In addition to being a natural antagonist of Activins, follistatin can inhibit the activity of other TGF-β ligands including BMPs, Myostatin, GDF-11, and TGF-β1. StemRD's Follistatin Fc fusion protein is full-length human Follistain-288 fused to the N-terminus of the Fc domain of human IgG1. This fusion allows stabilization of the protein in vitro and in vivo and dimerization of the recombinant protein rendering high-affinity binding to Activins. Expression is in human 293 cells cultured in serum-free and protein-free medium. Purification of the fusion protein from culture medium is achieved by using protein A chromatography.
• Purity: Greater than 95% as determined by SDS-PAGE analysis
• Biological Activity: The activity was determined by using a SMAD reporter gene assay in cultured human cells. The IC50 ranges from 20 - 100 ng/ml in the inhibition of 2 ng/mL Activin A activity
• For Research Use Only. Not for Use in Human
