Cellartis DEF-CS?500 iPS無(wú)外源3D懸浮培養(yǎng)基
傳統(tǒng)的2D培養(yǎng)系統(tǒng)無(wú)法支持放大培養(yǎng)（Scale-up）所需細(xì)胞數(shù)量級(jí)別（> 109個(gè)細(xì)胞/處理）以維持細(xì)胞療法的增長(zhǎng)需求。?3D懸浮培養(yǎng)是大規(guī)模生產(chǎn)多能細(xì)胞的可行經(jīng)濟(jì)有效的解決方案。盡管干細(xì)胞傳統(tǒng)上難以適應(yīng)懸浮培養(yǎng)，但CellArtis研究人員已經(jīng)開(kāi)發(fā)出一種培養(yǎng)體系，可以在成分確定、無(wú)飼養(yǎng)層和無(wú)異源物條件下實(shí)現(xiàn)穩(wěn)定高效的生長(zhǎng)并維持多能性。Cellartis DEF-CS?500 iPS無(wú)外源3D懸浮培養(yǎng)基與生物反應(yīng)器系統(tǒng)配套，為創(chuàng)建大量高質(zhì)量，未分化的起始材料提供了理想的方法。該培養(yǎng)基的配方基于2D培養(yǎng)基Cellartis DEF-CS 500 Xeno-Free Culture Medium，2D培養(yǎng)的細(xì)胞可以輕松過(guò)渡到3D以實(shí)現(xiàn)可靠的放大。
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英文名稱(chēng)：Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics
中文名稱(chēng)：Cellartis DEF-CS?500無(wú)外源3D懸浮iPS培養(yǎng)基，不含抗生素
用途：iPS?細(xì)胞3D懸浮細(xì)胞治療所需10億級(jí)
保存：基礎(chǔ)培養(yǎng)基2-8°C，添加劑-20°C
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組份
基礎(chǔ)培養(yǎng)基+三種添加劑
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics (Cat. No. Y30047)
500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o Antibiotics (Cat. No Y30046; not sold?separately)
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Additives (Cat. No. Y30048)
o 500 μl DEF-CS Xeno-Free 3D Spheroid Additive 1 (1,000X)
o 200 μl DEF-CS Xeno-Free 3D Spheroid Additive 2 (4,000X)
o 400 μl DEF-CS Xeno-Free 3D Spheroid Additive 3 (500X)
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自行購(gòu)買(mǎi)試劑
緩沖液和細(xì)胞分離試劑
PBS Dulbecco’s w/o Ca2+ & Mg2+ (D-PBS?–/–)
Accumax (Merck Millipore, Cat. No. SCR006)
可選抗生素和消化試劑
Gentamicin (Thermo Fisher, Cat. No. 15750)
Versene Solution (Thermo Fisher, Cat. No. 15040) (for transfer from monolayer culture)
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細(xì)胞培養(yǎng)參數(shù)
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細(xì)胞接種量
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錐形培養(yǎng)瓶大小
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培養(yǎng)體積
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1–4 x 106
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25 ml
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?5–15 ml
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3–8 x 106
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100 ml
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16–30 ml
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7–20 x 106
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250 ml
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35–65 ml
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Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium
Traditional 2D culture systems simply cannot support the scale-up required (>109 cells/treatment) to sustain the growing demand for cell-based therapies. 3D suspension culture is a viable, cost-effective solution for large-scale production of pluripotent cells. Although stem cells have traditionally been difficult to adapt to suspension culture, our scientists have developed a culture system that allows robust, stable growth and maintenance of pluripotency under defined, feeder-free, and xeno-free conditions. The Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium paired with a perfusion bioreactor system provides an ideal method for creating large amounts of high-quality, undifferentiated starting material. The formulation of this medium is based on the 2D medium, Cellartis DEF-CS 500 Xeno-Free Culture Medium, and cells growing in 2D can easily be transitioned to 3D for robust scale-up.
