英文名稱：Cellartis DEF-CS 500 Xeno-Free Culture Medium without Antibiotics
中文名稱：Cellartis DEF-CS?500?無外源?iPS?培養(yǎng)基，不含抗生素
特點(diǎn)：不含抗生素，無飼養(yǎng)層，非酶法單細(xì)胞傳代。
用途：臨床前級(jí)別iPS細(xì)胞生產(chǎn)
保存：基礎(chǔ)培養(yǎng)基2-8°C，添加劑-20°C
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Cellartis DEF-CS 500 Xeno-Free Culture Medium 不含抗生素，適用于在完全不含人和動(dòng)物來源組分的條件下擴(kuò)增人誘導(dǎo)多能干（iPS）細(xì)胞。細(xì)胞不使用飼養(yǎng)層細(xì)胞的情況下培養(yǎng)；而是使用諸如iMatrix-511的無異源物基底。培養(yǎng)方案使用溫和的非酶促單細(xì)胞傳代以確保可控制性和重復(fù)性。Cellartis DEF-CS?500無動(dòng)物源培養(yǎng)基（不含抗生素）中培養(yǎng)的細(xì)胞具有高增殖率和穩(wěn)定的核型，適合有效擴(kuò)增。這些特性的結(jié)合使得該培養(yǎng)基成為再生醫(yī)學(xué)研究臨床前級(jí)別iPS細(xì)胞生產(chǎn)的理想選擇。
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特點(diǎn)：
不含人類或動(dòng)物來源成分
優(yōu)化用于培養(yǎng)臨床前級(jí)人類iPS細(xì)胞
保留了iPS細(xì)胞的多能性和長(zhǎng)期的自我更新和分化潛能
提供高重復(fù)性和穩(wěn)定的生長(zhǎng)
使用單細(xì)胞傳代方法
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組份：基礎(chǔ)培養(yǎng)基+添加劑
Cellartis DEF-CS Xeno-Free Culture Medium without Antibiotics (Cat. No. Y30045)
500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium without Antibiotics (Cat. No Y30046; not?sold separately)
Cellartis DEF-CS 500 Xeno-Free Additives (Cat. No. Y30042)
o 500 μl DEF-CS Xeno-Free Additive 1 (1000X)
o 200 μl DEF-CS Xeno-Free Additive 2 (1000X)
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另行購買試劑：
緩沖液、培養(yǎng)基質(zhì)和消化劑
PBS Dulbecco’s（含鈣鎂和不含鈣鎂）和細(xì)胞培養(yǎng)基質(zhì)
Corning Synthemax II-SC Substrate (Corning, Cat. No. 3535) or iMatrix-511 (Takara Clontech, Cat. No. T303)
PBS Dulbecco’s with Ca2+ & Mg2+ (D-PBS +/+)
PBS Dulbecco’s w/o Ca2+ & Mg2+ (D-PBS –/–)
Versene Solution (Life Technologies, Cat. No. 15040)
Cellartis DEF-CS 500 Xeno-Free Culture Medium without Antibiotics is intended for the expansion of human induced pluripotent stem (iPS) cells in conditions that are completely free from human- and animal-derived components. Cells are cultured in the absence of feeder cells; instead, a xeno-free substrate such as iMatrix-511 is used. The culture protocol uses gentle, non-enzymatic single-cell passaging to ensure controlled and reproducible conditions. Cells cultured in Cellartis DEF-CS 500 Xeno-Free Culture Medium without Antibiotics have a high-proliferation rate and stable karyotype, allowing for efficient scale-up. The combination of these features makes this medium ideal for generating preclinical-grade iPS cells for regenerative medicine studies.
