產品編號：HY-001463

BioGems鼠β-NGF預包被ELISA試劑盒，Murine β-NGF Pre-Coated ELISA Kit

Murine β-NGF Pre-Coated ELISA Kit

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生產廠家： BioGems
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規(guī)格型號： 1 Kit
產品編號： HY-001463
產品種類： 細胞活力檢測

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產品描述 說明書【下載】用戶評論【0】

β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival.

The BioGems Murine β-NGF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure β-NGF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for β-NGF. It contains NS0-expressed recombinant β-NGF. The detection antibody is a biotinylated goat polyclonal antibody specific for β-NGF. The kit is analytically validated with ready to use reagents.

β-NGF是與BDNF，NT-3和NT-4蛋白結構相關的神經營養(yǎng)因子。這些蛋白屬于可能與穩(wěn)定二聚體結構相關的半胱氨酸結家族生長因子。 β-NGF是通過其受體β-NGFR傳導信號的強效神經營養(yǎng)因子，并且在感覺和交感神經系統(tǒng)的發(fā)育和保存中起關鍵作用。 β-NGF還充當B淋巴細胞的生長和分化因子，并增強B細胞存活。

BioGems鼠β-NGF預包被ELISA（酶聯(lián)免疫吸附試驗）試劑盒采用固相免疫測定試劑盒，專門設計用于檢測β-NGF，其中96孔微孔板預包被β-NGF特異小鼠單克隆抗體NGF。它含有NS0表達的重組β-NGF。檢測抗體是對β-NGF特異的生物素化山羊多克隆抗體。該試劑盒經過分析驗證，是即用型試劑。

Product Details

Assay Type:	Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:	<1pg/ml
Range:	31.2pg/ml-2000pg/ml
Specificity:	Natural and recombinant Murine β-NGF
Uniprot:	P01139
Interference:	No detectable cross-reactivity with other relevant proteins
Storage Conditions:	Store the unopened product at 2 – 8 ℃.

Components組份

Description	Quantity	Volume
Anti-Murine β-NGF Pre-coated 96-well strip microplate	1	12 strips of 8 wells
Murine β-NGF Standard	2	10 ng vial
Murine β-NGF Biotinylated antibody (100x)	1	130 μL
Avidin-Biotin-Peroxidase Complex (100x)	1	130 μL
Sample Diluent	1	30mL
Antibody Diluent	1	12mL
Avidin-Biotin-Peroxidase Diluent	1	12mL
Wash Buffer Concentrate (Powder for 1000mL)	1	Pack
Color Developing Reagent	1	10mL
Stop Solution	1	10mL
Plate Sealers	4	Each

Reproducibility?可重復性

?	Intra-Assay			Inter-Assay
Sample ID	1	2	3	1	2	3
n=	16	16	16	24	24	24
Mean Measured Concentration (pg/mL)	136	727	1326	153	839	1645
Standard Deviation	5.3	41.44	83.54	6.58	53.7	121.73
Consistency (%CV)	3.9	5.7	6.3	4.3	6.4	7.4

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