Catalog Number :BGK27548
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CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to “immunodeficiency with hyper-IgM”, a disease characterized by failure to produce IgG, IgA and IgE.
The BioGems Murine CD40 Ligand Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a?solid phase immunoassay specially designed to measure CD40 Ligand with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for CD40 Ligand. It contains NS0-expressed recombinant CD40 Ligand. The detection antibody is a biotinylated goat polyclonal antibody specific for CD40 Ligand. The kit is analytically validated with ready to use reagents.
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CD40是TNF受體家族的一員，是在B細(xì)胞，樹突狀細(xì)胞，單核細(xì)胞，胸腺上皮細(xì)胞上表達(dá)的細(xì)胞表面蛋白，在T細(xì)胞上表達(dá)水平低。CD40信號(hào)在B細(xì)胞的增殖和分化中起重要作用，并且對(duì)于免疫球蛋白（Ig）類型轉(zhuǎn)換（class switching）起關(guān)鍵作用。膜錨定的CD40配體幾乎完全只表達(dá)在活化的CD4 + T淋巴細(xì)胞上。未能表達(dá)CD40L會(huì)導(dǎo)致“高IgM免疫缺陷”，這是一種以未能產(chǎn)生IgG，IgA和IgE為特征的疾病。
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BioGems鼠CD40配體預(yù)包被ELISA（酶聯(lián)免疫吸附試驗(yàn)）試劑盒采用固相免疫測(cè)定法，專門設(shè)計(jì)用于檢測(cè)CD40配體，并在96孔微孔板上預(yù)包被CD40配體特異性小鼠單克隆抗體。它含有NS0表達(dá)的重組CD40配體。檢測(cè)抗體是CD40配體特異的生物素化山羊多克隆抗體。該試劑盒經(jīng)過(guò)分析驗(yàn)證，是即用型試劑。
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Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<10pg/ml
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Range:
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62.5pg/ml-4000pg/ml
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Specificity:
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Natural and recombinant Murine CD40 Ligand
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Uniprot:
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P27548
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 – 8 ℃.
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Components?組份
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Description
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Quantity
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Volume
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Anti-Murine CD40 Ligand Pre-coated 96-well strip microplate
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1
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12 strips of 8 wells
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Murine CD40 Ligand Standard
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2
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10 ng vial
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Murine CD40 Ligand Biotinylated antibody (100x)
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1
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130 μL
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Avidin-Biotin-Peroxidase Complex (100x)
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1
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130 μL
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Sample Diluent
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1
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30mL
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Antibody Diluent
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1
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12mL
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Avidin-Biotin-Peroxidase Diluent
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1
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12mL
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Wash Buffer Concentrate (Powder for 1000mL)
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1
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Pack
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Color Developing Reagent
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1
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10mL
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Stop Solution
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1
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10mL
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Plate Sealers
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4
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Each
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Reproducibility?可重復(fù)性
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Intra-Assay
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Inter-Assay
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Sample ID
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1
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2
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3
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1
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2
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3
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n=
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16
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16
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16
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24
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24
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24
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Mean Measured Concentration (pg/mL)
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537
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1562
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3025
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562
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1675
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3140
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Standard Deviation
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28.5
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71.9
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172.4
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36.53
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97.15
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207.24
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Consistency (%CV)
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5.3
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4.6
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5.7
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6.5
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5.8
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6.6
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