產品編號：HY-001460

BioGems鼠MIP-3β（CCL19）預包被ELISA試劑盒，Murine MIP-3β (CCL19) Pre-Coated ELISA Kit

Murine MIP-3β (CCL19) Pre-Coated ELISA Kit

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生產廠家： BioGems
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規(guī)格型號： 1 Kit
產品編號： HY-001460
產品種類： 細胞活力檢測

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Catalog Number :BGK70460

MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3β is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells.

The BioGems Murine MIP-3β (CCL19) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3β (CCL19) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3β (CCL19). It contains E. coli-expressed recombinant MIP-3β (CCL19). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3β (CCL19). The kit is analytically validated with ready to use reagents.

MIP-3β是一種CC趨化因子，在胸腺，淋巴結和活化的骨髓基質細胞中表達，通過CCR7受體傳導信號。MIP-3β是T和B淋巴細胞和骨髓祖細胞的趨化因子（chemoattractant）。

BioGems鼠MIP-3β（CCL19）預包被ELISA（酶聯(lián)免疫吸附測定）試劑盒采用固相免疫測定法，專門設計用于檢測MIP-3β（CCL19），其中96孔微孔板預包被小鼠MIP-3β特異性單克隆抗體（CCL19）。它含有大腸桿菌表達的重組MIP-3β（CCL19）。檢測抗體是對MIP-3β特異的生物素化山羊多克隆抗體（CCL19）。該試劑盒經過分析驗證，是即用型試劑。

Product Details

Assay Type:	Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:	<10pg/ml
Range:	15.6pg/ml-1000pg/ml
Specificity:	Natural and recombinant Murine MIP-3β(CCL19)
Uniprot:	O70460
Interference:	No detectable cross-reactivity with other relevant proteins
Storage Conditions:	Store the unopened product at ?2 – 8 ℃.

Components?組份

Description	Quantity	Volume
Anti-Murine MIP-3β?(CCL19) Pre-coated 96-well strip microplate	1	12 strips of 8 wells
Murine MIP-3β?(CCL19) Standard	2	10 ng vial
Murine MIP-3β?(CCL19) Biotinylated antibody (100x)	1	130 μL
Avidin-Biotin-Peroxidase Complex (100x)	1	130 μL
Sample Diluent	1	30mL
Antibody Diluent	1	12mL
Avidin-Biotin-Peroxidase Diluent	1	12mL
Wash Buffer Concentrate (Powder for 1000mL)	1	Pack
Color Developing Reagent	1	10mL
Stop Solution	1	10mL
Plate Sealers	4	Each

Reproducibility?可重復性

?	Intra-Assay			Inter-Assay
Sample ID	1	2	3	1	2	3
n=	16	16	16	24	24	24
Mean Measured Concentration (pg/mL)	91	286	592	105	379	645
Standard Deviation	3.82	14.3	34.93	6.51	28.05	52.25
Consistency (%CV)	4.2	5	5.9	6.2	7.4	8.1

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