神經(jīng)調(diào)節(jié)蛋白/調(diào)蛋白是由選擇性剪接基因（NRG1，NRG2，NRG3和NRG4）衍生的結(jié)構(gòu)相關(guān)多肽生長因子家族。迄今為止，有超過14種來源于NRG1基因的可溶性和跨膜蛋白?？缒?/span>NRG1同種型的胞外域的蛋白水解加工釋放可溶性生長因子。 HRG1-β1含有Ig結(jié)構(gòu)域和EGF樣結(jié)構(gòu)域;后者對于直接結(jié)合受體酪氨酸激酶erb3和erb4是必需的。該結(jié)合誘導(dǎo)erb3和erb4與erb2異源二聚化，刺激導(dǎo)致酪氨酸磷酸化的內(nèi)在激酶活性。

BioGems人類神經(jīng)調(diào)節(jié)蛋白預(yù)包被ELISA（酶聯(lián)免疫吸附測定）試劑盒是一種固相免疫測定，專門設(shè)計(jì)用于測量Neuregulin的96孔帶板，其預(yù)先包被有特異于神經(jīng)調(diào)節(jié)蛋白的小鼠單克隆抗體。它含有大腸桿菌表達(dá)的重組Neuregulin。檢測抗體是特異于Neuregulin的生物素化山羊多克隆抗體。該試劑盒已經(jīng)過分析驗(yàn)證，是即用型試劑。

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Product Details

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Components

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Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms releases soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain; the latter is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity that leads to tyrosine phosphorylation.

The BioGems Human Neuregulin Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Neuregulin with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for Neuregulin. It contains E. coli-expressed recombinant Neuregulin. The detection antibody is a biotinylated goat polyclonal antibody specific for Neuregulin. The kit is analytically validated with ready to use reagents.