MIP-3β是一種CC趨化因子，在胸腺，淋巴結(jié)和活化的骨髓基質(zhì)細(xì)胞中表達(dá)，通過CCR7受體傳導(dǎo)。 MIP-3β是T和B淋巴細(xì)胞和骨髓祖細(xì)胞的趨化因子。
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BioGems人MIP-3β（CCL19）預(yù)包被ELISA（酶聯(lián)免疫吸附測(cè)定）試劑盒采用固相免疫法，專門設(shè)計(jì)用于檢測(cè)MIP-3β（CCL19），96孔微板預(yù)包被MIP-3β特異鼠單克隆抗體（CCL19）。它含有大腸桿菌表達(dá)的重組MIP-3β（CCL19）。檢測(cè)抗體是MIP-3β特異生物素化山羊多克隆抗體（CCL19）。該試劑盒是即用型試劑盒，已經(jīng)過分析驗(yàn)證。
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Human MIP-3β (CCL19) Pre-Coated ELISA Kit分析
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Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<10pg/ml
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Range:
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15.6pg/ml-1000pg/ml
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Specificity:
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Natural and recombinant Human MIP-3β?(CCL19)
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Uniprot:
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Q6IBD6
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 – 8 ℃.
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Components組份
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Description
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Quantity
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Volume
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Anti-Human MIP-3β(CCL19) Pre-coated 96-well strip microplate
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1
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12 strips of 8 wells
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Human MIP-3β?(CCL19) Standard
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2
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10 ng vial
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Human MIP-3β?(CCL19) Biotinylated antibody (100x)
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1
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130 μL
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Avidin-Biotin-Peroxidase Complex (100x)
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1
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130 μL
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Sample Diluent
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1
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30mL
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Antibody Diluent
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1
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12mL
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Avidin-Biotin-Peroxidase Diluent
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1
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12mL
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Wash Buffer Concentrate (Powder for 1000mL)
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1
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Pack
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Color Developing Reagent
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1
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10mL
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Stop Solution
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1
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10mL
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Plate Sealers
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4
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Each
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Reproducibility?可重復(fù)性
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?
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Intra-Assay
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Inter-Assay
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Sample ID
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1
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2
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3
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1
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2
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3
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n=
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16
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16
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16
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24
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24
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24
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Mean Measured Concentration (pg/mL)
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97
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293
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583
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117
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374
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627
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Standard Deviation
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5.53
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14.1
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36.15
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7.5
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18.7
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42.64
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Consistency (%CV)
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5.7
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4.8
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6.2
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6.4
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5
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6.8
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Catalog Number : BGK6IBD6
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Description
MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3β?is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells.
The BioGems Human MIP-3β?(CCL19) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3β?(CCL19) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3β?(CCL19). It contains E. coli-expressed recombinant MIP-3β?(CCL19). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3β?(CCL19). The kit is analytically validated with ready to use reagents.
