APRIL是TNF超家族的一員，在單核細(xì)胞，巨噬細(xì)胞，某些轉(zhuǎn)化的細(xì)胞系，某些結(jié)腸癌和淋巴組織中表達(dá)。 APRIL與另一個(gè)TNF家族成員BAFF競(jìng)爭(zhēng)兩種受體，TACI和BCMA。 APRIL具有刺激各種腫瘤細(xì)胞系（包括Jurkat T細(xì)胞和MCF-7癌細(xì)胞）增殖的能力。像BAFF一樣，APRIL也刺激B和T細(xì)胞的增殖。人類APRIL基因編碼至少四種選擇性剪接的transcriptional variants，其產(chǎn)生APRIL前體蛋白的不同亞型。所有亞型都可以被furin蛋白酶剪切以釋放可溶性C末端片段，其包含APRIL前體的TNF樣受體結(jié)合位點(diǎn)。
BioGems人類APRIL預(yù)包被ELISA（酶聯(lián)免疫吸附測(cè)定）試劑盒是采用固相免疫測(cè)定法，專門(mén)設(shè)計(jì)用于檢測(cè)APRIL，其中96孔微板預(yù)包被APRIL特異小鼠單克隆抗體。它含有NS0表達(dá)的重組APRIL。檢測(cè)抗體是APRIL特異生物素化山羊多克隆抗體。該試劑盒是即用型試劑盒，已經(jīng)過(guò)分析驗(yàn)證。
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Human April Pre-Coated ELISA Kit分析
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產(chǎn)品參數(shù)Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<10pg/ml
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Range:
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156pg/ml-10,000pg/ml
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Specificity:
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Natural and recombinant Human APRIL
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Uniprot:
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Q2QBA2
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 – 8 擄C.
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組份Components
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Description
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Quantity
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Volume
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Anti-Human April Pre-coated 96-well strip microplate
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1
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12 strips of 8 wells
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Human April Standard
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2
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10 ng vial
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Human April Biotinylated antibody (100x)
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1
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130 μL
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Avidin-Biotin-Peroxidase Complex (100x)
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1
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130 μL
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Sample Diluent
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1
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30mL
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Antibody Diluent
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1
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12mL
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Avidin-Biotin-Peroxidase Diluent
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1
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12mL
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Wash Buffer Concentrate (Powder for 1000mL)
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1
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Pack
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Color Developing Reagent
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1
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10mL
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Stop Solution
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1
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10mL
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Plate Sealers
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4
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Each
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可重復(fù)性Reproducibility
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Intra-Assay
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Inter-Assay
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Sample ID
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1
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2
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3
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1
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2
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3
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n=
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16
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16
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16
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24
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24
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24
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Mean Measured Concentration (pg/mL)
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653
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2945
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5633
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864
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3214
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5366
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Standard Deviation
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26.8
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156.1
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219.7
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42.4
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202.5
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268.3
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Consistency (%CV)
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4.1
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5.3
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3.9
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4.9
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6.3
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5
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Catalog Number : BGK2QBA2
Description
APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of the colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, competes for two receptors, TACI and BCMA. APRIL has the ability to stimulate proliferation of various tumor cell lines, including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF-like receptor binding of the APRIL precursor.
The BioGems Human APRIL Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure APRIL with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for APRIL . It contains NS0-expressed recombinant APRIL . The detection antibody is a biotinylated goat polyclonal antibody specific for APRIL . The kit is analytically validated with ready to use reagents.
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