產(chǎn)品英文名稱：RetroNectin® Recombinant Human Fibronectin Fragment
產(chǎn)品中文名稱：重組人纖粘連蛋白
RetroNectin® 重組人纖粘連蛋白 T100B產(chǎn)品簡(jiǎn)介：
RetroNectin的試劑已被廣泛使用，以提高逆轉(zhuǎn)錄病毒和慢病毒基因轉(zhuǎn)移方法進(jìn)哺乳動(dòng)物細(xì)胞中。
此外，RetroNectin的試劑增強(qiáng)的T淋巴細(xì)胞的增殖。 T細(xì)胞通常被擴(kuò)大在白細(xì)胞介素-2（IL-2）的由刺激存在下與抗CD3抗體。在這種刺激步驟的加成RetroNectin的的顯著地增加了T細(xì)胞的擴(kuò)增效率。此外，T細(xì)胞擴(kuò)大了與該方法包含較少分化的T細(xì)胞（幼稚T細(xì)胞）的比例高。幼稚T細(xì)胞具有分化成細(xì)胞毒性T細(xì)胞在體內(nèi)的能力。
RetroNectin® 重組人纖粘連蛋白 T100B產(chǎn)品英文描述：
RetroNectin Reagent—Recombinant Human Fibronectin
Research using hematopoietic cells and other suspension cells has been limited in part by low efficiency of gene transfer (transduction and transfection) into these cell types. RetroNectin reagent promotes co-localization of lentivirus or retrovirus with target cells to dramatically enhance transduction efficiency. As a consequence, RetroNectin reagent is now the gold standard transduction enhancer for retroviral/lentiviral gene transfer to hematopoietic cells and holds great promise in clinical application, having been used in over 40 clinical trials.
RetroNectin reagent has been widely used to enhance retroviral and lentiviral gene transfer method into mammalian cells.
In addition, RetroNectin reagent enhances the proliferation of T lymphocytes. T cells are conventionally expanded in the presence of Interleukin-2 (IL-2) by stimulation with anti-CD3 antibody. The addition of RetroNectin in this stimulation step dramatically increases the efficiency of T cell expansion. In addition, T cells expanded with this method contain a high proportion of less-differentiated T cells (naive T cells). Naive T cells have the ability to differentiate into cytotoxic T cells in vivo.
Features
Enhanced gene transfer efficiency for retroviral or lentiviral transduction of suspension cells, hematopoietic stem cells, and other hard-to-infect cell types
Less toxicity than other commonly used transduction-enhancers, including polybrene and protamine
Fully recombinant peptide
Clinical grade RetroNectin (GMP) can be used for ex vivo clinical applications (investigational use only) - for GMP grade only please complete and return this form prior to purchase
Form :
Sterile solution containing 12.5 mM sodium citrate (pH6.2) and 1.25% sucrose
Source :
E. coli expressing human fibronectin fragment CH-296
Molecular weight :
62,617 Da (determined from amino acid sequence)
Storage : -20℃
Purity : > 90% by HPLC
RetroNectin® 重組人纖粘連蛋白 T100B相關(guān)產(chǎn)品訂購(gòu)信息：
Cat. # Product Size
T100B RetroNectin® Recombinant Human Fibronectin Fragment 2.5 mg
T110A RetroNectin® Precoated Dish 10 Dishes
T100A RetroNectin® Recombinant Human Fibronectin Fragment 0.5 mg
T201 RetroNectin® (GMP) 2.5 mg
