產(chǎn)品英文名稱：Ficoll PM400
產(chǎn)品中文名稱：聚蔗糖PM400

GE 聚蔗糖PM400（干粉）Ficoll PM400簡介：
高分子量的蔗糖聚合物由蔗糖與表氯醇共聚而成。該分子是高度支化的和羥基的高含量導(dǎo)致在含水介質(zhì)中的溶解性非常好。
為了生產(chǎn)密度梯度的細(xì)胞和亞細(xì)胞成分的離心或沉降在單位重力分離。
蔗糖的中性的，高度支化的，親水性聚合物，該聚合物容易溶解在水溶液中。
高達(dá)50％的濃度（重量/體積），覆蓋多達(dá)1.2克/毫升的密度，就可以實(shí)現(xiàn)。
更好的滲透性能比蔗糖。
保留功能和形態(tài)的完整性。
可用于分離細(xì)胞，離心敏感和（下沉降在單位重力條件下）分離密度相近，但不同大小的細(xì)胞。
不穿透生物膜。
作為原料制備的菲可 - 帕克梯度。
用在其它的應(yīng)用，如電泳，雜交，冷凍保存，并作為半抗原的載體。
Ficoll PM400是一種合成的中性的，高度支化的親水性的蔗糖為400000的平均分子量的聚合物，它一直被用來形成密度梯度分離和隔離真核細(xì)胞，細(xì)胞器和細(xì)菌細(xì)胞中，作為穩(wěn)定劑，并作為分離單核細(xì)胞制劑媒體。應(yīng)用程序也可以在限定的培養(yǎng)基，核酸雜交，電泳和免疫學(xué)研究發(fā)現(xiàn)。

GE 聚蔗糖PM400（干粉）Ficoll PM400英文描述：
Application： Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes.
It also has applications in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies.
Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity.
Density Max.：1.2 g/ml
Endotoxin Activity Max.：0.12 EU/mg
Estimated Shelf Life from Manufacture Date：5 years
Storage Conditions ：4 - 30°C
Stability：Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
Physical State：Solid
Physical Form：Spray-dried powder
Molecular Weight [Mr] Max.：~ 5 x 10~ 5
Molecular Weight [Mr] Min.：~ 3 x 105
Reactivity and Stability：The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, however, Ficoll PM 400 can be sterilized by autoclaving at 110°C for 30 min without degradation. Strong oxidizing and reducing agents should be avoided.
Stokes Radius：~ 10 nm
Color：White
Specific Rotation [alpha]20：50°-65°
Intrinsic Viscosity ：~ 17 ml/g
Solubility ：It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained