產(chǎn)品英文名稱(chēng)：RNA Polymerase II H14 Monoclonal Antibody
產(chǎn)品中文名稱(chēng)：RNA聚合酶II H14單克隆抗體

Covance RNA聚合酶II H14單克隆抗體 Polymerase II H14 Monoclonal Antibody產(chǎn)品英文描述：
Description ：Monoclonal Antibody against RNA Polymerase II
Also Known As ：DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; RNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit
Clone ：H14
Form ：Ascites
Host ：Mouse
Species Reactivity ;Extensive (Yeast to Human)
IsoType ：IgM
Specificity ：The antibody H14 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites; ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. This antibody recognizes the phosphoserine 5 version of pol II.
Uses ：This antibody is effective in immunoblotting, immunofluorescence (IF), immunoprecipitation (IP), pol II purification and functional studies.
Uses ：This antibody is effective in immunoblotting, immunofluorescence (IF), immunoprecipitation (IP), pol II purification and functional studies.
Suggested Working Dilution ;Quality control tests were done by Western and Dot blots. The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval.
Western blot: 1:500*
IF: 1:50-100
IP: 1:100
Notes ：*Predicted MW = 220-240 kD
*use casein or BSA in the blocking solution rather than non-fat-dry milk
Storage ：Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Covance RNA聚合酶II H14單克隆抗體使用注意事項(xiàng)：
建議工作稀釋度：質(zhì)量控制測(cè)試是由Western and Dot blots完成。最佳工作稀釋度應(yīng)為每個(gè)特定的實(shí)驗(yàn)條件來(lái)確定。這種抗體被出售用于實(shí)驗(yàn)室研究使用，不用于人類(lèi)或體內(nèi)使用?？莆乃箍贵w，不得轉(zhuǎn)售或修改轉(zhuǎn)售未經(jīng)事先書(shū)面批準(zhǔn)。
Western blot檢測(cè)：1:500*
IF：1:50-100
IP：1:100
注釋?zhuān)?預(yù)測(cè)分子量=220-240 kD的
*使用酪蛋白或BSA的封閉液，而不是非脂肪干乳
儲(chǔ)存：儲(chǔ)存于-20°C。經(jīng)初步解凍，分配到分裝并儲(chǔ)存于-20°C。但應(yīng)避免反復(fù)凍融，以防止變性的抗體。對(duì)于長(zhǎng)期儲(chǔ)存，保持抗體在-80°C。