產(chǎn)品英文名稱：RNA Polymerase II H5 Monoclonal Antibody
產(chǎn)品中文名稱：RNA聚合酶II H5單克隆抗體
Covance RNA聚合酶II H5單克隆抗體 Polymerase II H5 Monoclonal Antibody英文介紹：
Description;：?Monoclonal Antibody against RNA Polymerase II
Also Known As ?：DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; RNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit
Intended Use??：** **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use. **
Clone ?：H5
Form?：?Ascites
Host ?：Mouse
Species： Reactivity ?Extensive (Yeast to Human)
IsoType ：?IgM
Specificity ?：The antibody H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites; ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. This antibody recognizes the phosphoserine 2 version of pol II.
Uses ?：This antibody is effective in immunofluorescence (IF), immunoblotting (WB), immunoprecipitation (IP), pol II purification and functional studies.
Suggested Working Dilution ?：Quality control tests were done by Western blot and Dot blot. The optimal working dilution should be determined for each specific assay condition.
WB: 1:500*
IF: 1:50 – 1:100
IP: 1:100
Notes?：?*Predicted MW = 220-240 kDa
*use casein or BSA in the blocking solution rather than non-fat-dry milk
Storage ?：Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Covance RNA聚合酶II H5單克隆抗體使用注意事項(xiàng)：
建議工作稀釋度 質(zhì)量控制測(cè)試是由Western blot和Dot blot完成。最佳工作稀釋度應(yīng)為每個(gè)特定的實(shí)驗(yàn)條件來確定。
WB：1:500*
IF：1:50 – 1:100
IP：1:100
注: 預(yù)測(cè)分子量=220-240 kDa的
使用酪蛋白或BSA的封閉液，而不是非脂肪干乳。
儲(chǔ)存：儲(chǔ)存于-20°C。經(jīng)初步解凍，分配到分裝并儲(chǔ)存于-20°C。但應(yīng)避免反復(fù)凍融，以防止變性的抗體。對(duì)于長(zhǎng)期儲(chǔ)存，保持抗體在-80°C。