產(chǎn)品英文名稱(chēng):WNT-3a,Mouse
產(chǎn)品中文名稱(chēng):鼠重組WNT-3a蛋白

WNT-3a,Mouse 產(chǎn)品介紹:
wnt通路是細(xì)胞增殖分化的關(guān)鍵調(diào)控環(huán)節(jié)， 在胚胎發(fā)育和腫瘤發(fā)生中起著重要作用。wnt途徑參與了基因表達(dá)調(diào)節(jié)、 細(xì)胞遷移粘附、細(xì)胞極化等過(guò)程，同時(shí)還與其它信號(hào)通路存在交叉協(xié)同。Wnt/β-catenin通路在進(jìn)化過(guò)程中高度保守，此通路的主要分子構(gòu)成及相關(guān)調(diào)控機(jī)制已得到基本闡明。對(duì)神經(jīng)系統(tǒng)而言已有足夠證據(jù)顯示此通路參與了對(duì)神經(jīng)前體細(xì)胞增殖， 分化以及決定細(xì)胞命運(yùn)的調(diào)控。
Wnt-3a是Wnt基因家族中重要成員，最近的一些研究發(fā)現(xiàn)， Wnt-3a信號(hào)蛋白對(duì)神經(jīng)干細(xì)胞的定向分化的調(diào)控作用表現(xiàn)的十分明顯。小鼠WNT-3a蛋白包含328個(gè)氨基酸。

WNT-3a,Mouse產(chǎn)品特性:
? WNT-3a,Mouse活性經(jīng)過(guò)NIH 3T3 細(xì)胞鑒定，純度高
? 生物活性在NIH 3T3 細(xì)胞經(jīng)過(guò)TCF報(bào)告基因?qū)嶒?yàn)鑒定The EC50為 50–150 ng/ml
? WNT-3a,Mouse產(chǎn)品經(jīng)過(guò)SDS-PAGE 和HPLC雙層鑒定純度大于90%
? 只限于研究,非人體使用

WNT-3a,Mouse 產(chǎn)品英文簡(jiǎn)述:
WNT-3a is a member of the WNT family of signaling proteins that play a key role in embryonic development and the integrity of adult tissues. WNT-3a is a prototypic canonical WNT that signals through the β-catenin pathway. The predicted size of mouse WNT-3a is a monomeric protein containing 333 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of 40 kDa by SDS-PAGE analysis under nonreducing conditions. StemRD’s product is expressed from a mouse cell line overexpressing mouse WNT-3a. Purification is performed with a proprietary process that is distinct from the published method.

? Purity: Greater than 90% as determined by SDS-PAGE analysis
? Biological Activity: The activity was determined by using a TCF reporter gene assay in NIH3T3 cells. The EC50 ranges from 50 – 150 ng/ml. Country of Origin USA