產(chǎn)品英文名稱(chēng)：Miltenyi CD4+ T Cell Isolation Kit II, mouse
產(chǎn)品中文名稱(chēng)：美天旎小鼠CD4+ T細(xì)胞分離試劑盒II
Miltenyi 美天旎小鼠CD4+ T細(xì)胞分離試劑盒II英文描述：
PLEASE NOTE: The separation protocol is faster now! Download the updated datasheet.
The CD4+ T Cell Isolation Kit II was developed for the untouched isolation of CD4+ T cells from single-cell suspensions from mouse spleen or lymph nodes.
The kit is based on a cocktail of biotin-conjugated antibodies against CD8a, CD11b, CD11c, CD19, CD45R (B220), CD49b (DX5), CD105, Anti-MHC-class II, and Ter-119. The isolation of T cells is achieved by depletion of magnetically labeled cells.
Untouched T cells are used for functional studies in which cross-linking of surface molecules should be excluded. Isolated T cells can also be further magnetically separated into T cell subsets.
The CD4+ T Cell Isolation Kit has been used for the following applications: For adoptive transfer experiments1,2 , T cell proliferation assays1,2 , Coculture with dendritic cells3 and Cytokine analysis3 experiments.
美天旎小鼠CD4+ T細(xì)胞分離試劑盒II研究背景：
在CD4 + T細(xì)胞分離試劑盒II已發(fā)展為隔離從小鼠脾細(xì)胞或淋巴結(jié)中懸浮的CD4 + T細(xì)胞的結(jié)細(xì)胞。非CD4+ T細(xì)胞，即細(xì)胞毒性T細(xì)胞，B細(xì)胞，NK細(xì)胞，樹(shù)突細(xì)胞，巨噬細(xì)胞，粒細(xì)胞，內(nèi)皮細(xì)胞和紅系細(xì)胞通過(guò)間接唄磁性標(biāo)記的使用生物素標(biāo)記的抗體與抗生物素的混合物微珠。高純度的CD4 + T細(xì)胞的隔離是通過(guò)實(shí)現(xiàn)耗盡磁標(biāo)記的非目標(biāo)細(xì)胞。
Miltenyi 美天旎小鼠CD4+ T細(xì)胞分離試劑盒II產(chǎn)品介紹：
Components
1 mL CD4+ T Cell Biotin-Antibody Cocktail,mouse: Cocktail of biotin-conjugated monoclonal antibodies against CD8a, CD11b,CD11c, CD19, CD45R (B220), CD49b (DX5),CD105, Anti-MHC class II, and Ter-119.
2 mL Anti-Biotin MicroBeads:
MicroBeads conjugated to monoclonal antibiotin antibodies (isotype: mouse IgG1).
Capacity
For 10*9 total cells, up to 100 separations.
Product format
All components are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage
Store protected from light at 2 − 8 °C. Do not freeze. The expiration date is indicated on the vial label.
Miltenyi 美天旎小鼠CD4+ T細(xì)胞分離試劑應(yīng)用：
●CD4+ T細(xì)胞功能研究。
●研究CD4+ T細(xì)胞的活化信號(hào)的要求，誘導(dǎo)T細(xì)胞增殖，誘導(dǎo)T細(xì)胞無(wú)反應(yīng)性等等
●研究CD4+ T細(xì)胞的信號(hào)轉(zhuǎn)導(dǎo)。
●研究CD4+ T細(xì)胞細(xì)胞因子表達(dá)的調(diào)控。
作為Miltenyi 的代理商，華雅思創(chuàng)生物長(zhǎng)期備有現(xiàn)貨，價(jià)格優(yōu)惠。華雅思創(chuàng)生物以干細(xì)胞搜網(wǎng)為平臺(tái)，為用戶展示干細(xì)胞最全面的細(xì)胞培養(yǎng)耗材信息，期待您的訪問(wèn)。
