產(chǎn)品英文名稱：Miltenyi Stemgent Mouse Laminin  
產(chǎn)品中文名稱：美天旎Stemgent Pluriton小鼠層粘連蛋白

層粘連蛋白的作用：
層粘連蛋白是重組基底膜（RBM），分離出的小鼠Engelbreth-的Holm-群（EHS）腫瘤的主要成分。這種糖蛋白由包含促進(jìn)細(xì)胞附著和其它生物活性位點三種多肽鏈（A，B，和g）。胚胎干（ES）細(xì)胞表達(dá)的受體層粘連蛋白結(jié)合在本機(jī)層粘連蛋白分子的這些位點。采用層粘連蛋白作為生長基質(zhì)時，應(yīng)考慮存在RBM凝膠肽生長因子可能混淆實驗。以純的形式，層粘連蛋白足以支持優(yōu)異的ES細(xì)胞和iPS細(xì)胞生長中的未分化狀態(tài)中所定義的媒體使用和不使用的小分子激酶抑制劑。此外，層粘連蛋白可以作為一種替代的小鼠胚胎成纖維細(xì)胞（MEF）飼養(yǎng)細(xì)胞用于傳播多能干的人細(xì)胞系，有或無血清，并可以被用來支持推導(dǎo)和促進(jìn)譜系限制性祖細(xì)胞的分化。

Miltenyi Stemgent Mouse Laminin 小鼠層粘連蛋白產(chǎn)品介紹：
Size
1 mg
Concentration
1 mg/ml
Formulation
0.05 M Tris-HCl, pH 7.4, and 0.15 M NaCl
Source
Mouse EHS tumor
Purity
Greater than 98% pure by SDS-PAGE with no detectable entactin or proteoglycan (Figure 1).
Storage and Stability
Store the vial of Mouse Laminin at -20°C to -80°C. Mouse Laminin is stable for 3 months from date of receipt when stored as directed.
Thaw the vial of Mouse Laminin slowly on ice or at 4°C. Avoid repeated freeze-thaws as it can result in loss of activity.
Quality Control
Mouse Laminin is free of bacteria, fungi, mycoplasma, and murine specific viruses. Endotoxin level is less than 5 EU/ml. Mouse Laminin supports axonal outgrowth of dorsal root ganglion (DRG) as well as the attachment of mouse and human ES cells (Figure 2).

美天旎Stemgent Pluriton小鼠層粘連蛋白產(chǎn)品英文描述：
Part of the extracellular matrix (ECM) family of glycoproteins, laminin is an integral part of the structural scaffolding in most tissues1-2. In its pure form, laminin alone has proven to be sufficient in supporting embryonic stem (ES) and induced pluripotent stem (iPS) cell growth in the undifferentiated state in defined media with and without small molecule kinase inhibitors3. Laminin was first isolated from the mouse Engelbreth-Holm-Swarm (EHS) tumor, and is the main component of the reconstituted basement membrane (RBM)4. In addition, laminin can serve as an alternative to RBM for the attachment of pluripotent human cell lines with or without serum, and can be used to support derivation and promote the differentiation of lineage-restricted progenitor cells.

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