英文名稱：Recombinant Mouse Interferon-gamma
中文簡(jiǎn)稱：【mIFN g】重組小鼠干擾素-γ
Recombinant Mouse Interferon-gamma 【mIFN g】重組小鼠干擾素-γ 細(xì)胞因子產(chǎn)品描述：
干擾素（interferon,IFN）是細(xì)胞對(duì)相關(guān)刺激反應(yīng)時(shí)所產(chǎn)生的細(xì)胞信號(hào)蛋白質(zhì)，是細(xì)胞因子超家族中一個(gè)糖蛋白類同源細(xì)胞因子家族，稱為IFN家族。已鑒定出該家族中的3個(gè)主要成員：IFN-α、IFN-β和IFN-γ。其中干擾素-γ(Interferon-gamma，IFN-γ)是一種具有抗病毒、抗腫瘤和免疫調(diào)節(jié)作用的細(xì)胞因子，主要由活化的T細(xì)胞和NK細(xì)胞產(chǎn)生，在機(jī)體免疫系統(tǒng)中發(fā)揮重要作用。IFN-γ是一類分泌型糖蛋白，由143個(gè)氨基酸組成，相對(duì)分子量是16924。而重組人干擾素-γ相對(duì)分子量在14000-16000之間，這是由于重組IFN-γ在C末端有氨基酸丟失現(xiàn)象。
小鼠干擾素-γ重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有134個(gè)氨基酸，分子量為15601Da。該小鼠干擾素-γ重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主—大腸桿菌中重組表達(dá)
Synonyms?
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Introduction?
IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Description?
Interferon-gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6kDa.
The IFN-gamma is purified by proprietary chromatographic techniques.
Source?? （來(lái)源）
Escherichia Coli.
Physical Appearance? （物理特征）
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation?
Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4 and 5% trehalose.
Solubility?
It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18M?-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability? （穩(wěn)定性）
Lyophilized Interferon-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity?? （純度）
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence? （氨基酸序列）
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC.
Biological Activity? （生物活性）
The specific activity as determined in a viral resistance assay is < 0.1 ng/ml, corresponding to a specific activity of 1 x 107 Units/mg.
Recombinant Mouse Interferon-gamma 【mIFN g】重組小鼠干擾素-γ 細(xì)胞因子產(chǎn)品使用須知：
溶解：提供的小鼠干擾素-γ(mIFN g)產(chǎn)品為經(jīng)0.2um孔徑過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml小鼠干擾素-γ(mIFN g)的PBS（pH 7.4）溶液中過(guò)濾滅菌并凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：小鼠干擾素-γ(mIFN g)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
