英文名稱(chēng)：Recombinant Mouse Interleukin-6
中文簡(jiǎn)稱(chēng)：【mIL 6】重組小鼠白介素-6
Recombinant Mouse Interleukin-6 【mIL 6】重組小鼠白介素-6 細(xì)胞因子產(chǎn)品描述：
Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense，acute phase reactions，immune responses and hematopoiesis.
小鼠白介素-6重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有187個(gè)氨基酸，分子量為21709Da。該小鼠白介素-6重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---大腸桿菌中重組表達(dá)
Recombinant Mouse Interleukin-6 【mIL 6】重組小鼠白介素-6 細(xì)胞因子產(chǎn)品使用須知：
溶解：提供的小鼠白介素-6(mIL 6)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含小鼠白介素-6(mIL 6)的溶液（不含添加劑）中凍干產(chǎn)生的。我們建議加10mM HCl以配制工作儲(chǔ)存液到0.1-1.0 mg/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：小鼠白介素-6(mIL 6)在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Mouse Interleukin-6 【mIL 6】重組小鼠白介素-6 細(xì)胞因子產(chǎn)品英文描述：
Synonyms
IFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor.
Introduction
Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
Description
Interleukin-6 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton.
The IL-6 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance （物理特征）
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Solubility （溶解性）
It is recommended to reconstitute the lyophilized Mouse Il-6 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability （穩(wěn)定性）
Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity （純度）
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence （氨基酸序列）
FPTSQVRRGD FTEDTTPNRP VYTTSQVGGL ITHVLWEIVE MRKELCNGNS DCMNNDDALA ENNLKLPEIQ RNDGCYQTGY NQEICLLKIS SGLLEYHSYL EYMKNNLKDN KKDKARVLQR DTETLIHIFN QEVKDLHKIV LPTPISNALL TDKLESQKEW LRTKTIQFIL KSLEEFLKVT LRSTRQT.
Biological Activity （生物活性）
The ED50 as determined by the dose-dependant stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is < 0.02 ng/ml, corresponding to a specific activity of > 50,000,000 units/mg.
