英文名稱(chēng)：Recombinant Human Insulin Like Growth Factor-2
中文簡(jiǎn)稱(chēng)：【IGF2】重組人胰島素樣生長(zhǎng)因子-2
Recombinant Human Insulin Like Growth Factor-2 【IGF2】重組人胰島素樣生長(zhǎng)因子-2 細(xì)胞因子產(chǎn)品描述：
胰島素樣生長(zhǎng)因子2(IGF2)又稱(chēng)為生長(zhǎng)調(diào)節(jié)素A(somatomedin A)，是目前所知功能最復(fù)雜多樣的生長(zhǎng)因子。剛轉(zhuǎn)錄出來(lái)的原始IGF2是由156個(gè)氨基酸殘基組成的多肽鏈，經(jīng)過(guò)后續(xù)的剪切加工，成熟IGF2是一類(lèi)由67個(gè)氨基酸殘基組成的小分子弱酸性多肽，分子質(zhì)量7 471 u(不過(guò)在人的脊髓液中還發(fā)現(xiàn)一種9 ku的IGF2，在人腦中發(fā)現(xiàn)了更高分子質(zhì)量的IGF2，在人血清發(fā)現(xiàn)了一種15 ku的IGF2)。IGF2能促進(jìn)細(xì)胞的有絲分裂和分化，參與生后基因組重建，與X染色體的失活有重要關(guān)系。所有的這些功能說(shuō)明IGF2在機(jī)體生長(zhǎng)發(fā)育過(guò)程中的重要性。此外，IGF2與個(gè)體生長(zhǎng)速度、瘦肉率、背膘厚等生產(chǎn)性能關(guān)系密切，在某些物種已經(jīng)被作為候選基因確定下來(lái)。作為重要的印記基因，IGF2的表達(dá)狀況與腫瘤，以及潛在腫瘤的發(fā)生發(fā)展關(guān)系密切，在腫瘤治療方面起重要作用。
人胰島素樣生長(zhǎng)因子-2重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有67個(gè)氨基酸，分子量為7505Da。該人胰島素樣生長(zhǎng)因子-2重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---大腸桿菌中重組表達(dá)
Recombinant Human Insulin Like Growth Factor-2 【IGF2】重組人胰島素樣生長(zhǎng)因子-2 細(xì)胞因子產(chǎn)品產(chǎn)品須知：
溶解：提供的人胰島素樣生長(zhǎng)因子-2(IGF2)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含人胰島素樣生長(zhǎng)因子-2(IGF2)的溶液（不含添加劑）中凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：人胰島素樣生長(zhǎng)因子-2(IGF2)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Synonyms
Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734.
Introduction
IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Description
Insulin-Like Growth Factor-II Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7505 Dalton.
IGF-II is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance （外觀）
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized with no additives.
Solubility （溶解度）
It is recommended to reconstitute the lyophilized IGF2 in sterile 18MΩ-cm H2O at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability （穩(wěn)定性）
Lyophilized IGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFII should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity （純度）
Greater than 97.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence （氨基酸序列）
AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE.
Biological Activity （生物活性）
The ED50, calculated by the dose-dependent induction of MCF-7 cell proliferation was found to be 0.85ng/ml corresponding to a Specific Activity of 1,176,470IU/mg.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
