英文名稱：Recombinant Human Fibroblast Growth Factor-acidic
中文簡稱：【FGF 1】重組人酸性成纖維細胞生長因子

Recombinant Human Fibroblast Growth Factor-acidic 【FGF 1】重組人酸性成纖維細胞生長因子產(chǎn)品描述：

酸性成纖維細胞生長因子(acidic fibroblast growth factor，aFGF) 是成纖維細胞生長因子家族成員之一,對中胚層及神經(jīng)外胚層來源的細胞具有促分裂的作用。aFGF主要分布于大腦,它以自分泌或旁分泌的方式作用于周圍細胞,但其本身缺乏信號肽結構。aFGF蛋白由154個氨基酸殘基組成，等電點5～7，通過與其受體結合啟動信號轉(zhuǎn)導機制從而誘導多種細胞分化、增殖。
人酸性成纖維細胞生長因子重組蛋白是在大腸桿菌中重組表達的，是一條單鏈、非糖基化的多肽鏈，包含有141個氨基酸，分子量為15967Da。該人酸性成纖維細胞生長因子重組蛋白最終由標準的色譜技術純化出。
來源宿主---大腸桿菌中重組表達

Recombinant Human Fibroblast Growth Factor-acidic 【FGF 1】重組人酸性成纖維細胞生長因子產(chǎn)品使用須知：
溶解：提供的人酸性成纖維細胞生長因子(FGF 1)產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml人酸性成纖維細胞生長因子(FGF 1)的10mM 磷酸鈉（pH 7.5)和75mM NaCl溶液中過濾滅菌并凍干產(chǎn)生的。我們建議加無菌的4℃ 18MΩ-cm水以配制工作儲存液到0.1-0.25mg/ml。將樣本在4℃中豎直放置5分鐘后，離心去除沉淀物。
保存：人酸性成纖維細胞生長因子(FGF 1)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長保存時間，建議分裝后置于-18℃凍存。請避免反復凍融。

Recombinant Human Fibroblast Growth Factor-acidic 【FGF 1】重組人酸性成纖維細胞生長因子產(chǎn)品英文描述：

Synonyms 

HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.

Introduction 

Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.
Three alternatively spliced variants encoding different isoforms have been described.
The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Description 

Fibroblast Growth Factor-acidic Human Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of approximately 15.8kDa.
The FGF acidic is purified by proprietary chromatographic techniques.

Source 

Escherichia Coli.

Physical Appearance 

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing PBS, pH 7.4.

Solubility   （溶解性）

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile 18MΩ-cm H2O at 4 degrees Celsius at a concentration of 0.1mg-0.25mg per 1ml. Allow sample to sit for 5 min. at 4 degrees, spin to remove precipitant.

Stability    （穩(wěn)定性）

Lyophilized Fibroblast Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity      （純度）

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence    （氨基酸序列）

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Biological Activity     （生物活性）

The ED50, calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is <0.5 ng/ml, corresponding to a specific activity of > 2.0 x 1,000,000IU/mg.