英文名稱：Recombinant Human Keratinocye Growth Factor
中文簡(jiǎn)稱：【KGF】重組人角質(zhì)細(xì)胞生長(zhǎng)因子
Recombinant Human Keratinocye Growth Factor 【KGF】重組人角質(zhì)細(xì)胞生長(zhǎng)因子 描述：
人角質(zhì)細(xì)胞生長(zhǎng)因子重組蛋白為纖維母細(xì)胞生長(zhǎng)因子(FGF)的家庭。FGF家庭成員擁有廣泛的促有絲分裂細(xì)胞生存活動(dòng),并參與多種生物過(guò)程,包括胚胎發(fā)育、細(xì)胞生長(zhǎng)、形態(tài)發(fā)生、組織修復(fù)、腫瘤生長(zhǎng)和入侵。FGF7是一個(gè)強(qiáng)有力的上皮特異性生長(zhǎng)因子的促有絲分裂的活動(dòng)主要是表現(xiàn)在角質(zhì)細(xì)胞而不是在成纖維細(xì)胞和內(nèi)皮細(xì)胞。研究該基因的老鼠和老鼠同系物涉及角色在上皮的形態(tài)發(fā)生,reepithelialization傷口,頭發(fā)和早期肺器官發(fā)生發(fā)展。
人角質(zhì)細(xì)胞生長(zhǎng)因子重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有164個(gè)氨基酸，分子量為18995Da。該人角質(zhì)細(xì)胞生長(zhǎng)因子重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---大腸桿菌中重組表達(dá)
Recombinant Human Keratinocye Growth Factor 【KGF】重組人角質(zhì)細(xì)胞生長(zhǎng)因子 實(shí)驗(yàn)須知：
溶解：提供的人角質(zhì)細(xì)胞生長(zhǎng)因子(KGF)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml人角質(zhì)細(xì)胞生長(zhǎng)因子(KGF)的溶液（不含添加劑）中凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：人角質(zhì)細(xì)胞生長(zhǎng)因子(KGF)在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Human Keratinocye Growth Factor 【KGF】重組人角質(zhì)細(xì)胞生長(zhǎng)因子 英文描述：
Synonyms
HBGF-7, FGF7, FGF-7, KGF.
Introduction
KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
Description
Keratinocyte Growth Factor-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18995 Dalton.
The FGF-7 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2?m filtered solution in 20mM PB, pH 8.0, 1M NaCl.
Solubility
It is recommended to reconstitute the lyophilized Keratinocyte Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability (穩(wěn)定性）
Lyophilized Keratinocyte Growth Factor1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF7 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity （純度）
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence （氨基酸序列）
MCNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT.
Biological Activity (生物活性）
The biological activity was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing KGF receptors yielding an ED50 <10ng/ml, corresponding to a Specific Activity of 1.0×105 IU/mg.
Protein content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.9 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of KGF as a Reference Standard.
