英文名稱(chēng)：Recombinant Human Insulin Like Growth Factor-1
中文簡(jiǎn)稱(chēng)：【IGF1】重組人胰島素樣生長(zhǎng)因子-1
Recombinant Human Insulin Like Growth Factor-1 【IGF1】重組人胰島素樣生長(zhǎng)因子-1 細(xì)胞因子產(chǎn)品描述：
胰島索樣生長(zhǎng)因子一1(Insulin―like Growth Factor―l，IGF 1)是胰島素樣生長(zhǎng)因子的重要成員，與胰島索高度同源。這個(gè)系統(tǒng)的其它成員還包括IGF Ⅱ、1GF―l受體(IGFIR)、IGF Ⅱ受體(IGFIIR)以及4種胰島素生長(zhǎng)因子結(jié)合蛋白(IGF BP-1、-2、-3、-4)。其中胰島素樣生長(zhǎng)因子-1（Insulin-like growth factor-1，IGF-1）由70個(gè)氨基酸組成的單鏈堿性多肽分子，分子量為7649。它來(lái)源于體內(nèi)許多細(xì)胞，通過(guò)自分泌、旁分泌和內(nèi)分泌機(jī)制并與IGF-1受體（IGF-1R）結(jié)合發(fā)揮多種生理作用。作為一類(lèi)促進(jìn)細(xì)胞生長(zhǎng)、具有胰島素樣代謝效應(yīng)的因子。IGF-1受營(yíng)養(yǎng)狀態(tài)、激素、遺傳等多因素的調(diào)節(jié)，在心血管疾病、內(nèi)分泌代謝病及腫瘤等的病理生理過(guò)程中發(fā)揮重要作用。
人胰島素樣生長(zhǎng)因子-1重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有70個(gè)氨基酸，分子量為7655Da。該人胰島素樣生長(zhǎng)因子-1重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---大腸桿菌中重組表達(dá)。
Recombinant Human Insulin Like Growth Factor-1 【IGF1】重組人胰島素樣生長(zhǎng)因子-1 細(xì)胞因子產(chǎn)品實(shí)驗(yàn)須知：
溶解：提供的人胰島素樣生長(zhǎng)因子-1(IGF1)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含人胰島素樣生長(zhǎng)因子-1(IGF1)的溶液（不含添加劑）中凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：人胰島素樣生長(zhǎng)因子-1(IGF1)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Human Insulin Like Growth Factor-1 【IGF1】重組人胰島素樣生長(zhǎng)因子-1 細(xì)胞因子產(chǎn)品英文描述：
Synonyms
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Introduction
The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003).
Description
Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7kDa.
IGF-I is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized without any additives.
Solubility
It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE.
Amino acid sequence
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.
Biological Activity
The activity as determined by the dose-dependent proliferation of mouse FDC-P1 is less than 1.0 ng/ml, corresponding to a specific activity of 1x106 units/mg.
Protein content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard.
