英文名稱：Recombinant Human Interleukin-3
中文簡(jiǎn)稱：【IL 3 】重組人白介素-3
Recombinant Human Interleukin-3 【IL 3 】重組人白介素-3 細(xì)胞因子產(chǎn)品描述：
人白介素-3重組蛋白促進(jìn)細(xì)胞因子是一個(gè)強(qiáng)有力的增長(zhǎng)。這種細(xì)胞因子能夠支持廣泛的造血細(xì)胞的增殖。它是參與多種細(xì)胞活動(dòng),如細(xì)胞生長(zhǎng)、分化和凋亡。這種細(xì)胞因子也具有神經(jīng)營(yíng)養(yǎng)活性,而且它可能與神經(jīng)系統(tǒng)疾病有關(guān)。
人白介素-3重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有133個(gè)氨基酸，分子量為15000Da。該人白介素-3重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---大腸桿菌中重組表達(dá)
Recombinant Human Interleukin-3 【IL 3 】重組人白介素-3 細(xì)胞因子產(chǎn)品實(shí)驗(yàn)須知：
溶解：提供的人白介素-3(IL 3)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml人白介素-3(IL 3)的0.3 mg/ml NaHCO3 溶液中凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：人白介素-3(IL 3 )凍干粉末在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Human Interleukin-3 【IL 3 】重組人白介素-3 細(xì)胞因子產(chǎn)品英文描述：
Synonyms
MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.
Introduction
IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Description Interleukin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3.
Solubility
It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability （穩(wěn)定性）
Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity （純度）
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence （氨基酸序列）
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln.
Biological Activity The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000 IU/mg.
Protein content Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.
