產(chǎn)品英文名稱：R&D DG100 Human IGF-I Quantikine ELISA Kit
產(chǎn)品中文名稱：R&D DG100 人胰島素樣生長因子-I 定量酶聯(lián)免疫試劑盒

R&D DG100 Human IGF-I Quantikine ELISA Kit 產(chǎn)品簡介
R&D DG100 Human IGF-I Quantikine ELISA Kit 是一個3.5小時的固相酶聯(lián)免疫測定法是設(shè)計來檢測吸附在細(xì)胞培養(yǎng)物上清，血清和血漿中的人體IGF-I的。它包含大腸桿菌表達(dá)的重組人IGF-Ⅰ，并已被證明能準(zhǔn)確地定量重組因子。結(jié)果顯示，使用自然的人類IGF-I 獲得的平行曲線和使用Quantikine試劑盒標(biāo)準(zhǔn)得到的標(biāo)準(zhǔn)曲線呈線性。這些結(jié)果表明，本試劑盒可以被用來確定自然表達(dá)IGF-I的相對質(zhì)量值。

R&D DG100 人胰島素樣生長因子-I 定量酶聯(lián)免疫試劑盒產(chǎn)品參數(shù)
Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:3.5 hours
Sample Type & Volume Required Per
Well:Cell Culture Supernates (50 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL)
Sensitivity:0.056 ng/mL
Assay Range:0.094 - 6 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
Specificity:Natural and recombinant human IGF-I
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference:No significant interference observed with available related molecules.

R&D DG100 Human IGF-I Quantikine ELISA Kit 保存方法
Store the unopened kit at 2-8 °C. Do not use past kit expiration date.Store in a cool, dry place. Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.

R&D DG100 人胰島素樣生長因子-I 定量酶聯(lián)免疫試劑盒實(shí)驗操作程序
1.Prepare all reagents, standard dilutions, and samples as directed in the product insert.
2.Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
150 µL Assay Diluent

3.Add 150 µL of Assay Diluent to each well.
50 µL Standard, Control, or Sample

4.Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
5.Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
200 µL cold Conjugate

6. Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
7.Aspirate and wash 4 times.
200 µL Substrate Solution

8. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
50 µL Stop Solution

9.Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

R&D DG100 Human IGF-I Quantikine ELISA Kit 產(chǎn)品英文描述
R&D DG100 Human IGF-I Quantikine ELISA Kit is a 7.6 kDa, 70 amino acid (aa) polypeptide with threeinternal disulfide bonds. The sequence of human IGF-I is identical to that of bovine and porcineIGF-I, and it is 70% identical to human IGF-II. IGF-I is a single-chain molecule with about50% identity to the sequences of the A- and B-chains of human insulin. For reviews of IGF-Isee references 1-3.
While IGF-I has very complex functions, it appears to largely mediate the actions of growthhormone. Thus, IGF-I is important in prenatal development, growth to adulthood, and metabolic control. It induces amino acid uptake, protein synthesis, and glucose utilization. It is an important mitogen and regulator of the cell cycle and apoptosis. IGF-I is produced primarily by hepatocytes, serving an endocrine function. It is also produced by many other cells, where it may act in an autocrine or paracrine manner. Serum levels of IGF-I have been reported to increase from birth to puberty, followed by a slow decline through adulthood (4).
There are two receptors for the IGFs; type I IGF receptor, which signals through a tyrosine kinase, and type II IGF receptor, which is identical to the mannose-6-phosphate receptor and may not signal. The action of IGF on its receptors is very complex, with control by at least six IGF-binding proteins (IGFBP-1 through IGFBP-6). IGFBP-3 binds over 90% of the total IGF in serum in a complex of IGF, IGFBP, and an acid-labile subunit. This ternary complex greatly stabilizes IGF in the circulation, changing the half-life from minutes to hours. In addition,IGFBP's modulate the action of IGF on the membrane receptors. Adding to the complexity is a family of proteases that act on IGFBPs, modifying their affinity for IGF or completely eliminating the IGFBPs. The interactions of IGF, IGFBP, IGFBP proteases, and IGF receptors are referred to as the IGF axis. The IGF axis affects many primary physiological and pathological processes,including development, growth, metabolic regulation, tumorigenesis, atherosclerosis, and angiogenesis.
The Quantikine Human IGF-I Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-I in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that R&D DG100 Human IGF-I Quantikine ELISA Kit can be used to determine relative mass values for naturally occurring IGF-I.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.