產品英文名稱：Mouse IFN-γ ELISA Kit II
產品中文名稱：小鼠干擾素-γ ELISA 試劑盒 II

Mouse IFN-γ ELISA Kit II 產品介紹
Interferon-γ( IFN-γ ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors.1,2 In addition to its antiviral effects, IFN--γcan upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. IFN--γexerts strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN--γ’s capacity to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves. The BD Biosciences Pharmingen recombinant mouse IFN--γpresents as a 30 kD homodimeric protein, as shown by SDS-PAGE analysis.

Mouse IFN-γ ELISA Kit II 檢測規(guī)則
The BD OptEIA test is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for mouse IFN-? coated on a 96-well plate. Standards and samples are added to the wells, and any IFN-??present binds to the immobilized antibody. The wells are washed and a mixture of biotinylated anti-mouse IFN-??antibody and streptavidin-horseradish peroxidase is added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is added, which produces a blue color at levels in direct proportion to the amount of IFN-??present in the initial sample. The Stop Solution changes the color from blue to yellow, and the wells are read at 450 nm.

Mouse IFN-γ ELISA Kit II 保存方法
1. Store unopened kit at 2 – 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 – 25°C).
Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution,
use freshly reconstituted standard within 12 hours (stored at 2 – 8°C).

Mouse IFN-γ ELISA Kit II 的優(yōu)越性
•高靈敏度
•高通量，高含量的分析
•最小體積的生物材料的要求
•適用于冷凍/解凍的生物樣品 •與其他檢驗方法兼容。例如，細胞分析。BD™ ELISPOT可以轉移克隆，增殖實驗，流式細胞術或其他分析方法。

Mouse IFN-γ ELISA Kit II 使用注意事項
1. Reagents that contain preservatives may be toxic if ingested, inhaled, or come in contact with the skin.
2. Avoid contact of skin, eyes, or clothing with Stop Solution or Substrate Reagents.
3. Handle all serum and plasma specimens in accordance with NCCLS guidelines for preventing transmission of blood-borne infections.
4. TMB One-Step Substrate Reagent is toxic if inhaled or swallowed. Avoid contact with skin. Keep container tightly closed. In case of an accident or if you feel unwell, seek medical advice immediately.
5. Standard/Sample Diluent and ELISA Diluent contain 0.09% sodium azide. Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid
accumulation of potentially explosive deposits in plumbing.
6. The Wash Concentrate contains 16 % Sodium Chloride and is an irritant.
7. The Stop Solution contains 11.5% Phosphoric Acid and is a corrosive solution.