產(chǎn)品英文名稱：NS/0 HCP ELISA kit
產(chǎn)品中文名稱：NS/0 HCP ELISA 試劑盒

NS/0 HCP ELISA kit 的應(yīng)用
本試劑盒是用于確定在NS/0 的重組表達(dá)產(chǎn)品細(xì)胞中NS/0 宿主細(xì)胞蛋白污染的存在。該試劑盒是用于研究和制造使用，不用于人類或動(dòng)物的診斷應(yīng)用。

NS/0 HCP ELISA 試劑盒的英文描述
Hybridization of antibody producing cells with NS/0 cells is one of the most commonly employed systems for the production of humanized and mouse monoclonal antibodies. This kit has been successfully qualified for a number of different therapeutic human monoclonal antibodies. Our assay validation has determined that the LOD for this kit is ~300pg/mL.

NS/0 HCP ELISA kit 的使用步驟規(guī)則
The NS/0 assay is a two-site immunoenzymetric assay. Samples containing NS/0 HCPs are reacted with a horseradish peroxidase (HRP) enzyme labeled anti-NS/0 antibody in a test tube or simultaneously in microtiter strips coated with an affinity purified capture anti-NS/0 antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of NS/0 HCPs present.

NS/0 HCP ELISA 試劑盒的保存和穩(wěn)定性
1 All reagents should be stored at 2℃ to 8C℃for stability until the expiration date printed.
2 The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.
3 Reconstituted wash solution is stable until the expiration date of the kit.

NS/0 HCP ELISA 試劑盒的使用注意事項(xiàng)
1 只用于研究或制造。
2 停止反應(yīng)試劑是0.5N 硫酸。避免接觸眼睛，皮膚，和服裝
3 本試劑盒所用的標(biāo)準(zhǔn)包含來(lái)自人血清免疫球蛋白。源材料進(jìn)行了測(cè)試，發(fā)現(xiàn)有艾滋病和乙型肝炎表面抗原的抗體。沒有任何已知的方法可以使HIV或肝炎病毒或其他病原體總保證提供，因此處理這些試劑，好像他們是潛在的傳染。在用本試劑盒的試劑濃度，其他沒有被認(rèn)為是有害的。
4 本試劑盒只能由合格的技術(shù)人員使用。