產品英文名稱：R-spondin-1, human
產品中文名稱：人重組R-spondin-1蛋白

R-spondin-1, human產品介紹：
R-spondin-1是權威WNT信號通路的天然增強劑。當與激活β-catenin信號通路的WNT蛋白聯(lián)合使用時，R-spondin-1能通過與LGR5和LGR4融合而有效提高權威WNT蛋白的活性。
向小鼠注射重組R-spondin-1蛋白可以激活β-catenin信號通路和腸道下皮細胞的增殖，為臨床改善化療誘導結腸炎操作提供了基礎和依據。
全片段R-spondin-1的預測分子量是25.6kDa，而且在特異性條件下，由于SDS-PAGE葡糖基化的影響，分子量可達到約39kDa。StemRD公司的R-spondin-1是由人293細胞作為分泌蛋白，再由一系列色譜分析法純化而來。

R-spondin-1, human產品英文簡述：
R-spondin-1 is a natural enhancer of the canonical WNT pathway. When used together with WNT proteins that activate the beta-catenin pathway, Rspondin-1 enhances the activity of canonical WNT proteins by binding to LGR5 and LGR4 (refs.) Injection of recombinant R-Spondin-1 into mouse causes activation of the-catenin pathway and proliferation of intestinal crypt cells, which forms the basis for a clinical trial in amelioration of chemotherapy-induced colitis. The Predicted MW of full-length R-Spondin-1 is 25. 6 kDa, and due to glycosylation it runs on SDS-PAGE at ~39 kDa under reducing conditions. StemRD’s R-Spondin-1 is produced in human 293 cells as a secreted protein (without tag) and purified by a series of chromatography.

• Purity：Greater than 90% as determined by SDS-PAGE analysis
• Biological Activity：The activity was determined by using a TCF reporter gene assay in cultured
human cells. The EC50 ranges from 5 - 20 ng/ml in the presence of 10ng/mL human WNT-3a. Activity in other assays should be determined by each individual setting
• For Research Use Only. Not for Use in Human