產品英文名稱：Noggin,human
產品中文名稱：人Noggin蛋白

Noggin,human 產品介紹：
Noggin 被認為是同時對BMP及抗BMP生物活性物質均具有高親和性的結合蛋白中的一種。在胚胎干細胞培養(yǎng)過程中加入Noggin，可以有效提高干細胞的多能性。
重組的人Noggin蛋白在進行移動單一多肽鏈處理后共包含216個氨基酸，并且達到預測的分子量24.6kDa。 糖基化反應后，隨著反應條件的變化，Noggin逐漸集結成SDS-PAGE從而達到大約30kDa的分子量。
StemRD公司生產的Noggin來源于經(jīng)過翻譯后特異性修飾的人293細胞，且經(jīng)過一系列的色譜分析方法對產品進行純化。

Noggin,human產品特性：
» SDS-PAGE檢測純度大于95%
» 生物活性在人293細胞中用受體基因檢測確定，EC50在25 - 100 ng/mL之間
» Noggin,human只限于科學研究，非人體使用

Noggin,human 產品英文簡述：
Noggin has been shown to be a high-affinity binding protein to BMP and antagonizes BMP bio-activities. It can be added to cell culture medium for embryonic stem cells to maintain pluripotency.
The recombinant human Noggin comprises 216 amino acids after removal of the signal peptide and has a predicted molecular mass of 24.6 kDa. As aresult of glycosylation, it migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
StemRD's Noggin is made in human 293 cells, which renders humanspecific post-translational modifications. We purified this product through a series of chromatography.

• Purity：Greater than 95% by SDS-PAGE
• Biological Activity：The activity was determined by using a reporter gene assay in human 293 cells for the inhibition of activity of BMP4 (2.5ng/mL). The EC50 ranges from 25 - 100 ng/mL
• For Research Use Only. Not for Use in Humans