Murine MIP-3β (CCL19) Pre-Coated ELISA Kit
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Catalog Number :BGK70460
MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3β is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells.
The BioGems Murine MIP-3β (CCL19) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3β (CCL19) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3β (CCL19). It contains E. coli-expressed recombinant MIP-3β (CCL19). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3β (CCL19). The kit is analytically validated with ready to use reagents.
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MIP-3β是一種CC趨化因子,在胸腺,淋巴結(jié)和活化的骨髓基質(zhì)細(xì)胞中表達(dá),通過(guò)CCR7受體傳導(dǎo)信號(hào)。MIP-3β是T和B淋巴細(xì)胞和骨髓祖細(xì)胞的趨化因子(chemoattractant)。
BioGems鼠MIP-3β(CCL19)預(yù)包被ELISA(酶聯(lián)免疫吸附測(cè)定)試劑盒采用固相免疫測(cè)定法,專門設(shè)計(jì)用于檢測(cè)MIP-3β(CCL19),其中96孔微孔板預(yù)包被小鼠MIP-3β特異性單克隆抗體(CCL19)。 它含有大腸桿菌表達(dá)的重組MIP-3β(CCL19)。 檢測(cè)抗體是對(duì)MIP-3β特異的生物素化山羊多克隆抗體(CCL19)。該試劑盒經(jīng)過(guò)分析驗(yàn)證,是即用型試劑。
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Product Details
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Assay Type: |
Solid Phase Sandwich ELISA: 96-Well Strip Plate |
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Sensitivity: |
<10pg/ml |
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Range: |
15.6pg/ml-1000pg/ml |
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Specificity: |
Natural and recombinant Murine MIP-3β(CCL19) |
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Uniprot: |
O70460 |
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Interference: |
No detectable cross-reactivity with other relevant proteins |
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Storage Conditions: |
Store the unopened product at ?2 – 8 ℃. |
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Components?組份
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Description |
Quantity |
Volume |
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Anti-Murine MIP-3β?(CCL19) Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
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Murine MIP-3β?(CCL19) Standard |
2 |
10 ng vial |
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Murine MIP-3β?(CCL19) Biotinylated antibody (100x) |
1 |
130 μL |
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Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 μL |
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Sample Diluent |
1 |
30mL |
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Antibody Diluent |
1 |
12mL |
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Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
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Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
|
Color Developing Reagent |
1 |
10mL |
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Stop Solution |
1 |
10mL |
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Plate Sealers |
4 |
Each |
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Reproducibility?可重復(fù)性
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? |
Intra-Assay |
Inter-Assay |
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Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
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n= |
16 |
16 |
16 |
24 |
24 |
24 |
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Mean Measured Concentration (pg/mL) |
91 |
286 |
592 |
105 |
379 |
645 |
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Standard Deviation |
3.82 |
14.3 |
34.93 |
6.51 |
28.05 |
52.25 |
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Consistency (%CV) |
4.2 |
5 |
5.9 |
6.2 |
7.4 |
8.1 |
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