產(chǎn)品編號(hào)：HY-001459

BioGems 人RANTES 預(yù)包被ELISA試劑盒,Human RANTES Pre-Coated ELISA kit

Human RANTES Pre-Coated ELISA kit

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生產(chǎn)廠家： BioGems
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規(guī)格型號(hào)： 1 Kit
產(chǎn)品編號(hào)： HY-001459
產(chǎn)品種類： 細(xì)胞活力檢測(cè)

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RANTES是CC趨化因子，可通過CCR1，CCR3，CCR5和US28（巨細(xì)胞病毒受體）受體傳導(dǎo)信號(hào)。它是一種趨化單核細(xì)胞，記憶T細(xì)胞（CD4 + / CD45RO），嗜堿性粒細(xì)胞和嗜酸性粒細(xì)胞的趨化劑。RANTES還具有抑制某些HIV-1，HIV-2和猿免疫缺陷病毒（SIV）的能力。

BioGems人類RANTES預(yù)包被ELISA（酶聯(lián)免疫吸附測(cè)定）試劑盒采用固相免疫測(cè)定法，專門設(shè)計(jì)用于檢測(cè)RANTES，96孔板預(yù)包被RANTES特異性小鼠單克隆抗體。它含有大腸桿菌表達(dá)的重組RANTES。檢測(cè)抗體是RANTES特異性的生物素化山羊多克隆抗體。該試劑盒已經(jīng)過分析驗(yàn)證，是即用型試劑。

Product Details

Assay Type:	Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:	<3pg/ml
Range:	15.6pg/ml-1000pg/ml
Specificity:	Natural and recombinant Human Rantes
Uniprot:	D0EI67
Interference:	No detectable cross-reactivity with other relevant proteins
Storage Conditions:	Store the unopened product at 2 – 8 ℃.

Components

Description	Quantity	Volume
Anti-Human Rantes Pre-coated 96-well strip microplate	1	12 strips of 8 wells
Human Rantes Standard	2	10 ng vial
Human Rantes Biotinylated antibody (100x)	1	130 μL
Avidin-Biotin-Peroxidase Complex (100x)	1	130 μL
Sample Diluent	1	30mL
Antibody Diluent	1	12mL
Avidin-Biotin-Peroxidase Diluent	1	12mL
Wash Buffer Concentrate (Powder for 1000mL)	1	Pack
Color Developing Reagent	1	10mL
Stop Solution	1	10mL
Plate Sealers	4	Each

Reproducibility

?	Intra-Assay			Inter-Assay
Sample ID	1	2	3	1	2	3
n=	16	16	16	24	24	24
Mean Measured Concentration (pg/mL)	147	293	501	110	339	658
Standard Deviation	5.88	15.53	31.1	8.14	24.41	56.6
Consistency (%CV)	4	5.3	6.2	7.4	7.2	8.6

Description

RANTES is a CC chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV).

The BioGems Human RANTES Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure RANTES with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for RANTES. It contains E. coli-expressed recombinant RANTES. The detection antibody is a biotinylated goat polyclonal antibody specific for RANTES. The kit is analytically validated with ready to use reagents.

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