上皮調(diào)節(jié)蛋白是與EGFR（ErbB1）和ErbB4特異性結(jié)合但不結(jié)合ErbB2或ErbB3的EGF相關(guān)生長(zhǎng)因子。 它主要表達(dá)于胎盤(pán)和外周血白細(xì)胞，以及膀胱，肺，腎和結(jié)腸的某些癌癥中。上皮調(diào)節(jié)蛋白刺激角質(zhì)形成細(xì)胞，肝細(xì)胞，成纖維細(xì)胞和血管平滑肌細(xì)胞的增殖。它還抑制幾種腫瘤來(lái)源的上皮細(xì)胞系的生長(zhǎng)。
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BioGems Human Epiregulin Pre-Coated ELISA（酶聯(lián)免疫吸附測(cè)定）試劑盒是采用固相免疫測(cè)定法，專(zhuān)門(mén)設(shè)計(jì)用于檢測(cè)Epiregulin，96孔微板上預(yù)包被Epiregulin特異性小鼠單克隆抗體。 它含有大腸桿菌表達(dá)的重組Epiregulin。 檢測(cè)抗體是對(duì)Epiregulin特異的生物素化山羊多克隆抗體。該試劑盒已經(jīng)過(guò)分析驗(yàn)證，是即用型試劑。
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Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<10pg/ml
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Range:
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156pg/ml-10,000pg/ml
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Specificity:
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Natural and recombinant Human Epiregulin
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Uniprot:
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O14944
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 – 8 ℃.
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Components
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Description
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Quantity
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Volume
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Anti-Human Epiregulin Pre-coated 96-well strip microplate
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1
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12 strips of 8 wells
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Human Epiregulin Standard
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2
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10 ng vial
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Human Epiregulin Biotinylated antibody (100x)
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1
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130?μL
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Avidin-Biotin-Peroxidase Complex (100x)
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1
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130 μL
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Sample Diluent
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1
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30mL
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Antibody Diluent
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1
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12mL
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Avidin-Biotin-Peroxidase Diluent
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1
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12mL
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Wash Buffer Concentrate (Powder for 1000mL)
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1
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Pack
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Color Developing Reagent
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1
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10mL
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Stop Solution
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1
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10mL
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Plate Sealers
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4
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Each
|
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Reproducibility
|
?
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Intra-Assay
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Inter-Assay
|
|
Sample ID
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1
|
2
|
3
|
1
|
2
|
3
|
|
n=
|
16
|
16
|
16
|
24
|
24
|
24
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Mean Measured Concentration (pg/mL)
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1.15
|
2.52
|
5.81
|
1.63
|
2.87
|
5.66
|
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Standard Deviation
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0.059
|
0.113
|
0.273
|
0.096
|
0.146
|
0.35
|
|
Consistency (%CV)
|
5.2
|
4.5
|
4.7
|
5.9
|
5.1
|
6.2
|
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Description
Epiregulin is an EGF-related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not ErbB2 or ErbB3. It is expressed mainly in the placenta and peripheral blood leukocytes, as well as in certain carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. It also inhibits the growth of several tumor-derived epithelial cell lines.
The BioGems Human Epiregulin Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Epiregulin with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for Epiregulin. It contains E. coli-expressed recombinant Epiregulin. The detection antibody is a biotinylated goat polyclonal antibody specific for Epiregulin. The kit is analytically validated with ready to use reagents.
