產品英文名稱：Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit
產品中文名稱：Stemcell 19757 EasySep™ 小鼠乳腺干細胞富集試劑盒

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 產品簡介
Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit旨在從新鮮解離的小鼠乳腺組織中分離出乳腺干細胞。不需要的細胞被針對非上皮細胞(CD45, CD31, TER119, BP-1)的生物素化的抗體瞄準去除。標記的細胞然后被確認針對抗生物素和葡聚糖的四聚體的抗體配合物識別。這些細胞被綁定到磁性粒子并使用沒有柱的EasySep™磁鐵分離。未受破壞的上皮細胞富集餾分傾到一個新的管中，然后用CD24和CD49f抗體標記，這樣，CD24+CD49f++ 子種群就可以通過熒光激活細胞分選分離。

Stemcell 19757 EasySep™ 小鼠乳腺干細胞富集試劑盒產品組分
Stemcell 19757 EasySep™ 小鼠乳腺干細胞富集試劑盒有五個部分
• EasySep™ Mouse Epithelial Cell Enrichment Cocktail, 0.5 mL
• EasySep™ Biotin Selection Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 1.0 mL
• CD24 Antibody, Clone M1/69, PE-Conjugated, 0.5 mL
• CD49f Antibody, Clone GoH3, FITC-Conjugated, 1.0 mL

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 推薦使用
Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit is designed for the isolation of mouse mammary stem cells from freshly dissociated mammary tissue by negative selection
Stemcell 19757 EasySep™ 小鼠乳腺干細胞富集試劑盒所需設備
• Collagenase/Hyaluronidase Solution (Catalog #07912)
• Gentle Collagenase/Hyaluronidase Solution (Catalog #07919)

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 保存方法
EASYSEP™ MOUSE EPITHELIAL CELL ENRICHMENT COCKTAIL
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
EASYSEP™ BIOTIN SELECTION COCKTAIL
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
EASYSEP™ MAGNETIC NANOPARTICLES
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
ANTI-MOUSE CD24-PE ANTIBODY
Product stable at 2 - 8°C until expiry date as indicated on label. Store in the dark. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
ANTI-CD49f-FITC ANTIBODY
Product stable at 2 - 8°C until expiry date as indicated on label. Store in the dark. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 實驗資料
FACS Profile Results with EasySep™ Mouse Mammary Stem Cell Enrichment Kit

Freshly dissociated mouse mammary epithelial cells are distributed according to their expression of CD49f and CD24. The mammary repopulating unit (MRU)-rich fraction is found in the CD49f++CD24+ subset. The mammary colony-forming cells (Ma-CFCs) are found in the CD49f+ CD24++ subset. In certain strains of mice (such as C57BI/6J) there can be a small number of CD24++CD49f++ cells (indicated * in the dot plot). Although mammary stem cells have been detected in this fraction, their frequency is much lower than in the MRU fraction.

Typical EasySep™ Procedure for Mouse Cells


Stemcell 19757 EasySep™ 小鼠乳腺干細胞富集試劑盒相關產品訂購信息
Stemcell  07912  Collagenase/Hyaluronidase  10X Collagenase/Hyaluronidase in DMEM 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet
Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator 
Stemcell  05610  EpiCult™-B Mouse Medium Kit  Medium for Mouse Mammary Epithelial Cell Culture      
For research use only. Not intended for human or animal diagnostic or therapeutic uses.